Scientific Core: Bacillus anthracis Anthrax Toxin Core

炭疽毒素的作用和使用毒素成分作为炭疽疫苗的潜力， 这是该提案中许多拟议项目的核心。这个核心将为研究人员提供高度 纯化的炭疽毒素蛋白质，可用于大规模和小规模的研究一致。毒素核心 将净化大量的PA，LF和EF，即不含LPS。每批毒素将由核心进行测试， 将提供毒素的相对活性和标准化批次，以便研究人员进行多项研究 在这个项目的五年里，我们对这种试剂的一致性充满信心。该核心还将存储和 目录PA，LF和EF，并雇用一名技术人员，负责监督纯化，表征和 毒素的分布。

The role of anthrax toxin and the potential for using components of the toxin as an anthrax vaccine, are central to many of the proposed projects in this proposal. This core will provide investigators with highly purified anthrax toxin proteins that can be used consistently in large and small-scale studies. The toxin core will purify large amounts of PA, LF, and EF, that is LPS-free. Batches of toxin will be tested by the core for relative activity and normalized batches of toxin will be provided, so investigators can pursue multiple studies over the five years of this project confident in the consistency of this reagent. This core will also store and catalog PA, LF, and EF and employee a technician that will oversee the purification, characterization, and distribution of the toxin.