Rocky Mountain Neurological Disorders Core

项目总结（见说明）： 这是落基山神经疾病中心（RMNOC）的竞争性更新，其主要目标是加强和进一步研究NINOS针对的神经生物学领域。RMNO中心核心已经建立了设施，允许有效地生成和使用显示有趣和信息功能或行为表型的转基因动物。我们现有的核心都非常 成功的和发展，使国家的最先进的方法，从而允许用户以有效的方式进行合作研究。因此，我们建议保留原始内核的原始名称，并强调它们将在未来五年内继续发展，如申请中所述（内核A，6，C和0）。和以前一样，四个核心提供的服务在Anschutz的其他地方是不可用的。 医学院。 我们建议RMNO中心核心的四个核心设施： 核心A显微镜核心（比尔贝茨，主任）将提供先进的光学和电子显微镜服务。 核心B基因靶向/病毒载体核心（Mark Dell'Acqua，总监）\N\设计和构建载体以产生基因靶向和转基因小鼠以及病毒靶向载体。 Core C Zebrafish Transgenic Core（Angeles Ribera，Director）可提供用于维持和表征转基因斑马鱼的服务。 Core D Machine Shop Core（Ulli Bayer，董事）将提供机加工车间支持服务。

PROJECT SUMMARY (See instructions): This Is a competitive renewal of the Rocky Mountain Neurological Disorder Center (RMNOC) whose main goal Is to enhance and further research in areas of neurobiology targeted by the NINOS. The RMNO Center Core has established facilities that allow efficient generation and use of genetically-altered animals that display Interesting and Informative functional or behavioral phenotypes. Our existing cores have all been extremely successful and evolved to enable state-of-the-art approaches thereby allowing users to perform collaborative research in an efficient manner. Therefore, we propose to maintain the original cores with their original names, emphasizing that they will continue to evolve over the next five years, as described in the application (Cores A, 6, C, and 0). As before, the services provided by the four cores are not available elsewhere at the Anschutz Medical Campus. We propose four core facilities for the RMNO Center Core: Core A Microscopy Core (Bill Betz, Director) will provide advanced light and electron microscopy services. Core B Gene-Targeting/Viral Vector Core (Mark Dell'Acqua, Director) \N\\\ design and construct vectors to generate gene-targeted and transgenic mice as well as viral targeting vectors. Core C Zebrafish Transgenic Core (Angeles Ribera, Director)m\\ provide services for maintaining and characterizing transgenic zebraflsh. Core D Machine Shop Core (Ulli Bayer, Director) will provide machine shop support services.