Genomics - Shared Services

项目总结(见说明)： 基因组学共享服务(GSS)是一个全面的资源，用于支持马里兰大学医学院的研究人员进行基因组研究，包括Marlene和Stewart Greenebaum癌症中心(UMGCC)。服务包括DNA测序、基因表达研究、基因分型和高通量DNA测序。GSS提供了对必要的信息学资源的访问，以提供从实验计划和原始数据的初始分析到 生物学背景下的高级分析。GSS由两个与基因组相关的核心实验室组成，由第三个资源--信息学资源中心提供支持。由GSS运营的仪器是最先进的。 DNA测序服务由六台应用生物系统公司型号3730XL自动测序仪提供支持。 高通量DNA测序是在两台罗氏454FLX焦解测序仪、一台LLUMINA基因组分析仪IIX和一台LLUMINA HiSeq2000上进行的。基因分型和基因表达服务由应用生物系统型号7900 HT快速实时聚合酶链式反应系统、llLumina BeadXpress、Affymetrix基因芯片系统3000 7G和llLumina iScan支持。高技能的信息学教职员工提供 UMGCC的研究人员拥有使用现有软件工具分析多维数据集的专业知识，或根据需要开发新的、新颖的软件和算法。GSS的一个关键优势是向学生(研究生和医学)、研究员(博士后和临床)以及教职员工提供教育机会。这些教育机会对GSS在改变癌症研究项目方面的成功有着深远的影响。这一共享服务的成效可以通过每年支持的大量出版物来衡量--仅UMGCC成员每年就有100多份出版物。其中许多出版物都发表在影响力很大的期刊上，包括《细胞》、《 美国国家科学院、科学、自然遗传学、癌症研究、生物化学和分子生物学杂志。

PROJECT SUMMARY (See instructions): The Genomics Shared Service (GSS) Is a comprehensive resource for supporting genomic studies to investigators at the University of Maryland School of Medicine, including the Marlene and Stewart Greenebaum Cancer Center (UMGCC). Services include DNA sequencing, gene expression studies, genotyping, and high-throughput DNA sequencing. GSS provides access to the informatics resources necessary to provide a seamless pathway from experimental planning and initial analysis of raw data to advanced analysis in a biological context. GSS comprises two genomic-related core laboratories supported by a third resource, the informatics resource center. Instrumentation operated by the GSS is state-of-the-art. DNA sequencing services are supported by six Applied Biosystems model 3730XL automated sequencers. High-throughput DNA sequencing is performed on two Roche 454 FLX pyrosequencers, an lllumina Genome Analyzer IIX, and an lllumina HiSeq2000. Genotyping and gene expression services are supported by an Applied Biosystems model 7900 HT Fast real-time PCR system, and lllumina BeadXpress, and Affymetrix GeneChip system 3000 7G, and an lllumina iScan. Highly skilled informatics faculty and staff provide investigators at the UMGCC with the expertise to analyze multidimensional datasets, using existing software tools, or to develop new, novel software and algorithms as necessary. A key strength of the GSS is the educational opportunities provided to students (graduate and medical) and fellows (postdoctoral and clinical) as well as faculty. These educational opportunities have a profound impact on the success the GSS has in transforming cancer research programs. The effectiveness of this shared service can be measured by the large number of publications supported annually¿over 100 publications per year from members of the UMGCC alone. Many of these publications are in high-impact journals, including Cell, Proceedings of the National Academy of Sciences, Science, Nature Genetics, Cancer Research, Biochemistry, and the Journal of Molecular Biology.