Flow Cytometry Core Facility

流式细胞仪核心为中心成员提供流式细胞仪研究支持。核心致力于提供高质量的多色分析、高速分类(包括生物危害分类)、教育、咨询、应用开发和数据分析。自2003年审查以来，核心发生了巨大变化。使用量增加了50%。为了适应和允许这种增长，核心增加了硬件和工作人员。我们增加了四台分析细胞仪，三台9色蓝光和一台18色LSRII，以及两台分选机，一台数字MoFlo，XDP和反射。为BSL-2+分类配置的反射已放置在专门翻新的BSL-2+空间中。我们还将FACSCalibur和FACScan升级为双激光五色仪器。我们增加了两个高技能的1.00FTE职位，以适应增加的使用和提供额外的服务。值得注意的是，Core的工作人员总共有77年的流式细胞仪经验。 总之，该设施提供最先进的分析和分类流式细胞仪、专门知识、教育、咨询、数据分析和访问的便利性(域名和网站、中央数据存储和存档、基于网络的日程安排)。流式细胞术核心由拉里·阿诺德博士领导，他自1982年核心成立以来一直担任主任。 2009年，152个实验室使用了Core；中心成员占总使用量的76%。协调委员会的预算申请为141,810美元，占预计预算总额的23%。所要求的增加是为了帮助建立新的生物危害分类设施，该设施将首次允许中心成员对可存活的人体材料和其他含有病原体的材料进行分类。我们的未来 计划集中于对用户的教育，以提高他们的能力，从而提高数据质量。我们的单一技术投资计划是评估Amny ImageStream成像流式细胞仪技术对我们用户的实用性。

The Flow Cytometry Core provides flow cytometry research support for Center members. The Core endeavors to provide high quality multi-color analysis, high-speed sorting (including biohazard sorting), education, consultation, application development, and data analysis. The Core has changed dramatically since the 2003 review. Use has increased 50%. To accommodate and permit this increase the Core has increased both hardware and staff. We have added four analytical cytometers ~ three 9-color CyAns and an 18-color LSRII and two sorters ~ a digital MoFlo XDP and Reflection. The Reflection which is configured for BSL-2+ sorting has been placed in specially renovated BSL-2+ space. We have also upgraded the FACSCalibur and FACScan to dual-laser, 5-color instruments. We have added two highly skilled 1.00 FTE positions to accommodate the increased use and to provide additional services. Notably, the Core's staff has an aggregate of 77 years of flow cytometry experience. In sum the Facility provides state-of-the-art analytical and sorting flow cytometers, expertise, education, consultation, data analysis, and ease of access (domain and website, central data storage and archiving, web-based scheduling). The Flow Cytometry Core is directed by Dr. Larry Arnold, the Director since the inception of the Core In 1982. In 2009 152 laboratories used the Core; Center members accounted for 76% of total use. The CCSG budget request is for $141,810 representing a CCSG contribution of 23% to the total projected budget. The requested increase is to help establish the new biohazard sorting facility which for the first-time will permit sorting of viable human materials and other pathogen containing materials by Center members. Our future plans are to concentrate on education of users to increase their competency and, thus, data quality. Our single technology investment plan is to evaluate the utility of the Amnis ImageStream imaging flow cytometry technology for our users.