Hopkins Digestive Diseases Basic and Translational Research Core Center

霍普金斯消化疾病基础和翻译研究核心中心是一个拟议的Silvio Conte核心中心，旨在促进约翰霍普金斯大学医学院的基础科学和翻译消化疾病研究。这个核心中心的主题是“上皮功能的调节，特别是通过信号转导、运输和发育的变化”。它有一个由38个正式成员和12个准成员组成的研究基地。它由4个科学核心和1个管理核心组成。核心A，蛋白质组学核心将帮助确定在胃肠道/肝脏正常或在疾病中差异表达的蛋白质，蛋白质之间的相互作用，包括识别结合伙伴，将绘制切割位点图，并确定翻译后修饰。核心B，成像核心将为核心中心的研究人员提供多种类型的上皮细胞的尖端成像和EM(扫描、传输、免疫)，并提供一名核心经理来帮助执行研究。这些包括具有FRAP、FRET和TIRF的共焦和双光子显微镜；荧光计的使用；单细胞定量成像；数码相机；使用激光捕获显微镜。核心C，小鼠生理学核心将在以下方面提供建议：建立小鼠群体；对消化疾病的小鼠模型进行基因分型；提供代谢笼并进行尿液、粪便、血液采集和分析。该中心还将对小鼠进行主动脉灌注，作为肠道、肝脏、胰腺、肾脏采集的一部分，并为Northern和Western分析和组织切片准备做准备。一名小鼠病理学家、一名细胞因子多重ELISA和一名FACS技术人员是Core C的新成员。Core C使设备可用，并为Ussing电压室/电压钳研究提供使用指导。翻译研究增强核心帮助形成临床数据库和患者的DNA、RNA、标本采购样本采购管理核心将提供核心中心的沟通、财务管理和行政职能，组织丰富计划并管理试点项目和小型休假计划。

The Hopkins Digestive Diseases Basic and Translational Research Core Center is a proposed Silvio Conte Core Center which has been designed to advance basic science and translational digestive diseases research at the Johns Hopkins University School of Medicine. This Core Center has as its theme, "Regulation of Epithelial Function, Especially via Changes in Signal Transduction, Trafficking and Development". It has a Research Base of 38 full Members and 12 Associate Members. It consists of: 4 scientific Cores and an Administrative Core. Core A, Proteomics Core will help identify proteins that are differentially expressed in GI tract/liver normally or in diseases, interactions among proteins including identification of binding partners, will map cleavage sites and determine post-translational modifications. Core B, Imaging Core will make multiple types of cutting edge imaging and EM (scanning, transmission, immuno) of epithelial cells available to Core Center investigators and provide a Core Manager to help perform the studies. These include confocal and two-photon microscopy with FRAP, FRET and TIRF; use of fluorometers; single cell quantitative imaging; digital camera; access to Laser Capture Microscopy. Core C, Mouse Physiology Core will provide advice in establishing mouse colonies; genotype mouse models of digestive diseases; make available metabolic cages and perform urine, stool, blood collections and analysis. This Core will also perform aortic perfusion of mice as part of intestinal, liver pancreas, kidney procurement and preparation for Northern and Western analysis and histological slide preparation. A mouse pathologist, cytokine multiplex ELISA and a FACS technician are new components of Core C. Core C makes equipment available and provides instruction in its use for Ussing chamber/voltage clamp studies. Core D, Translational Research Enhancement Core provides help in forming a clinical database and specimen collection from patients for DNA, RNA, specimen procurement Administrative Core will provide the communication, financial management and administrative functions of the Core Center, organize the Enrichment Program and administer the Pilot Project and Mini-sabbatical Programs.