Core C - Flow Cytometry Core

项目总结（见说明）： 流式细胞术核心将是拟议的RDRCC的一个集成和重要组成部分。流动核心将支持风湿性疾病和其他相关免疫/炎症性疾病的前沿工作，包括免疫介导的肺损伤，脆弱的免疫学，速发型超敏反应以及细菌和病毒引起的慢性疾病。总体目标是提供细胞分选和多色流式细胞术 为一系列研究项目提供服务，包括人类风湿性疾病样本的复杂免疫表型分析、人类疾病中抗原驱动反应的研究、影响免疫细胞分化和功能的信号的基础研究以及人类疾病动物模型的开发。在未来五年的RDRCC中，我们提出了以下三个目标 细胞计数核心： 1.为RDRCC研究者提供最先进的流式细胞术仪器和方法。这包括随时获得技术、专门知识和培训。 2.提供一系列基于流式细胞术的程序，以增强和扩展RDRCC研究人员的研究计划。 3.吸引新的调查员利用流程核心，为此提供一个合议环境，开展有针对性的外联活动，并为各级调查员提供教育机会。

PROJECT SUMMARY (See instructions): The Flow Cytometry Core will be an integrated and essential component of the proposed RDRCC. The flow core will support cutting edge work on rheumatic diseases and other related immune/inflammatory disorders including immune mediated lung injury, immunology of frailty, immediate hypersensitivity and bacterial and viral induced chronic disease. The overall goal is to provide cell sorting and polychromatic flow cytometry services for a range of research initiatives including complex immune phenotyping of samples from human rheumatic disorders, investigations on the antigen driven response in human disease, basic studies on the signals that influence Immune cell differentiation and function and the development of animal models of human disease. In the next five years of the RDRCC we propose the followings three aims for the Flow Cytometry core: 1. Provide RDRCC investigators access to state of the art flow cytometry instrumentation and methodology. This includes ready access to technology, expertise and training. 2. Provide a range of flow cytometric based procedures that will enhance and expand the research programs of RDRCC investigators. 3. Attract new investigators to utilize the flow core by providing a collegial environment, through targeted outreach and by providing educational opportunities for investigators at all levels.