Beta & Gamma Oral HPV infection in HIV positive individuals

摘要： -- 感染艾滋病毒会增加机会性感染和癌症的易感性。口服药和口服药的风险 口咽癌在HIV血清阳性患者中增加，反映口腔人类 乳头瘤病毒(HPV)感染。来自多项研究的证据表明，口腔中含有过多的 以前与皮肤肿瘤有关的HPV种类，包括贝塔型和伽马-HPV型。我们 最近，L S发现，口腔中检测到的贝塔和伽马-人乳头瘤病毒与 口腔癌和喉癌的风险。这表明贝塔和伽马之间存在病因学联系- 口腔中的HPV类型，特别是在感染艾滋病毒的人群中，他们患上HPV的风险更高 发展各种由病毒引起的头颈部肿瘤。然而，到目前为止，人们对 HIV阳性或阴性患者口腔感染贝塔和伽马-HPV型的自然病史 人口。我们将利用现有NIDCR资助的研究中的临床基础设施，以及 与密歇根大学头颈孢子艾滋病毒补充财团合作扩大 NIDCR项目，测试从660个样本中收集的反复口腔冲洗、唾液和刷子(DNA和RNA)样本 下一代HIV阳性和HIV阴性患者对多种β和γ-HPV型的检测 测序。研究参与者被前瞻性地跟踪最长3年的时间，并完成 详细的流行病学调查，包括与吸烟、饮酒和 性行为。控制HIV感染、口腔损害和其他慢性疾病病史的临床资料 感染被收集起来。利用这些纵向数据，我们建议：1)评估和比较HPV类型 HIV感染者口腔内β、γ-HPV型分布及病毒活化(表达) 艾滋病毒阳性和阴性个体，以及2)确定和比较发病率和持久性，以及风险 HIV阳性和HIV阴性人群口腔中HPV感染的影响因素 个人。在这个项目完成后，我们将有准确的估计发病率和持久性 口腔β-和伽马-HPV感染，并确定艾滋病毒偶发感染和持续感染的危险因素- HIV阳性和HIV阴性的个人。此外，这项研究将对更大规模的规划具有重要意义 协作性研究，如通过头颈部孢子间联盟，评估自然 HIV感染人群中贝塔和伽马-HPV的病史及其与口腔癌风险的关系。

SUMMARY:   Infection with HIV increases susceptibility to opportunistic infections and cancer. The risk of oral and oropharyngeal carcinomas is increased in HIV-seropositive patients reflecting a higher rate of oral human papillomavirus (HPV) infection. Evidence from multiple studies indicates that the oral cavity contains a plethora of HPV species including beta and gamma-HPV types, previously associated with skin neoplasias. We recently a l s o found that detection of beta and gamma-HPV in the oral cavity was associated with increased risks of incident oral and laryngeal cancers. This suggests an etiologic association for beta and gamma- HPV types in the oral cavity, especially among HIV-infected populations who are at increased risk of developing a variety of virally induced head and neck tumors. However, to date, very little is known about the natural history of oral infection with beta and gamma-HPV types in either HIV-positive or HIV-negative populations. We will leverage the clinical infrastructure from an existing NIDCR funded study, and a collaboration with the University of Michigan Head and Neck SPORE HIV consortium supplement expanding the NIDCR project, to test repeated oral rinse, saliva and brush (DNA and RNA) samples collected from 660 HIV-positive and HIV-negative patients for multiple beta and gamma-HPV types by next-generation sequencing. Study participants are followed prospectively for a period of up to 3 years, and complete detailed epidemiologic surveys consisting of questions related to tobacco smoking, alcohol consumption and sexual practices. Clinical data related to control of HIV infection, oral lesions and history of other chronic infections are collected. Using this longitudinal data, we propose to: 1) assess and compare HPV type distribution and viral activation (expression) for beta- and gamma-HPV types in the oral cavity among HIV- positive and HIV-negative individuals, and 2) determine and compare the incidence and persistence of, and risk factors for, beta- and gamma-HPV infections in the oral cavity among HIV-positive and HIV-negative individuals. At the completion of this project, we will have precise estimates of the incidence and persistence of oral beta- and gamma-HPV infections, and identified risk factors for incident and persistent infection in HIV- positive and HIV-negative individuals. In addition, this study will be important for planning of larger collaborative studies, such as through the Head and Neck Inter-SPORE consortium, to assess the natural history of beta and gamma-HPV and their association with oral cancer risk in HIV-infected populations.