Genomics Core

JABSOM基因组学核心设施为夏威夷群岛的研究人员提供基因组服务，实验建议和数据分析。到目前为止，我们已经处理了来自多个地点的66多名个人用户的样本，包括Chaminade大学，UH Manoa，UH Hilo，JABSOM，Queens和Leahi医院以及癌症研究中心。自2009年3月创建核心项目以来，发票总额，无论是数量还是金额，都逐年稳步增长。我们的qPCR、测序和微阵列杂交的主要服务每年都在稳步上升。对成本和再充电回收的仔细评估表明，随着核心区活动的持续适度增长，我们可以在5年后实现收支平衡的财政业绩。The Core由一位才华横溢的经理Steffen Oeser管理，他曾在Illumina从事基因组技术工作8年。除了基因组服务，核心还向用户提供教育，并保持其工作人员的技能。为了在COBRE资助的这个周期中加强核心的产品，我们与弗吉尼亚生物信息学研究所建立了合作关系。提供复杂的信息学专业知识应该会增加核心产品的价值，特别是微阵列数据。我们也有一个创新的核心组成部分，主要是由一个新的助理教授和COBRE年轻的研究员，乍得沃尔顿的项目提供。这些包括配体选择的生物信息学方法，通过梯度“熔化”对SNP鉴定的潜在改进，以及场效应晶体管的工作，这将使我们能够测试配体预测算法的能力，以产生一种与肌钙蛋白结合的肽，用于诊断心脏损伤。过渡期COBRE支持将坚定地建立这一核心，作为对疾病分子理解所必需的关键基因组服务的可持续资源。

The JABSOM Genomics Core Facility offers genomic services, experimental advice, and data analysis to researchers of the Hawaiian Islands. To date we have processed samples from over 66 individual users from multiple sites including Chaminade University, UH Manoa, UH Hilo, JABSOM, Queens and Leahi Hospitals, and the Cancer Research Center. Total invoices, in both number and dollar amount, have steadily increased on a yearly basis since the creation of the Core in March of 2009. Our major services of qPCR, Sequencing, and Microarray Hybridization are each trending steadily upwards each year. A careful evaluation of costs and recharge recovery suggests that with continued moderate growth of the activity in the Core we can achieve break-even fiscal performance after 5 years. The Core is run by a talented Manager, Steffen Oeser, who worked for 8 years at lllumina in genomic technologies. In addition to genomic services the Core provides education to users and maintains the skills of its staff. In order to enhance the offerings of the Core in this cycle of COBRE funding we have established a collaboration with the Virginia Bioinformatics Institute. The provision of sophisticated informatic expertise should increase the value of the Core offerings, especially microarray data. We also have a component of innovation in the Core, provided largely by projects of a new Assistant Professor, and COBRE young investigator, Chad Walton. These include a bioinformatic approach to ligand selection, a potential improvement in SNP identification by a gradient "melt-off", and work on a field-effect transistor that will allow us to test the capability of the ligand-prediction algorithm to generate a peptide that will bind to troponin for diagnosis of cardiac damage. Transitional COBRE support will firmly establish this core as a sustainable resource for crucial genomic services that are necessary for a molecular understanding of disease.