Enhancing the whole genome sequence(WGS)based surveillance of Salmonella, Listeria and Shiga Toxin producing Escherichia coli(STEC) isolated from food and environment at the national and global level

从零售肉类、新鲜农产品及其 环境被认为是细菌性食源性疾病的主要原因 在美国和世界各地。重要的是，我们要监测患病率， 食品中重要食源性细菌菌株的趋势， 沿着从农场到餐桌的链条， 冲击因此，必须扩大现有的监测网络， 这些重要的病原体在全球范围内，以防止未来的爆发。我们提出 与世卫组织-AGISAR（综合咨询委员会）合作开展这项工作， 抗生素耐药性监测），资助各国进行监测 人类、食品和食品中的耐药性食源性细菌病原体 环境保护我们坚信，通过拟议的 工作将协助监测沙门氏菌，李斯特菌和STEC从国际 目前的监控系统还不够强大，无法追踪他们。的 WGS数据和元数据将显著提高解决疫情的能力， 帮助快速连接临床和零售产品样本，并协助疫情爆发 调查事务所PD（Dr. Thakur）实验室与NC部门合作 农业和消费者服务部（NCDACS）是GenomeTrakr的一个合作伙伴， 实验室我们的建议将大大有助于我们跟踪潜在的 新的沙门氏菌，李斯特菌和STEC菌株，并允许公共卫生机构采取 采取适当步骤，在全球一级保障人类和动物健康。 我们建议的具体目标是： 1)建立基于全基因组序列的强有力的监测系统 从食品和环境中分离的400株沙门氏菌、李斯特菌和STEC的概况 在美国和美国以外的来源。 2)比较全球范围内沙门氏菌、李斯特菌和STEC的WGS图谱， 协助疫情调查，并跟踪新出现的具有公共卫生重要性的菌株。 我们建议的长远目标，是透过以下措施，保障和促进公众健康 加强对沙门氏菌、李斯特菌和产肠毒素大肠杆菌的监测， 通过生成关键的WGS数据并为GenomeTrakr使命做出贡献，

Salmonella, Listeria and STEC isolated from retail meat, fresh produce and their environment have been implicated as the leading causes of bacterial foodborne illness in the US and around the world. It is important that we monitor the prevalence and trends of important foodborne bacterial strains in food products that can eventually pass to the consumers along the farm-to-fork chain resulting in significant public health impact. It is, therefore, important to expand the current surveillance network and monitor these important pathogens at the global scale to prevent future outbreaks. We propose to do this in collaboration with the WHO-AGISAR (Advisory Committee on Integrated Surveillance of Antimicrobial Resistance) which funds countries to conduct surveillance of antimicrobial resistant foodborne bacterial pathogens in humans, food products and the environment. We strongly believe that the data generated through the proposed work will assist in monitoring Salmonella, Listeria and STEC from at the international level where current surveillance systems are not robust enough to track them. The WGS data and metadata will significantly improve the power to resolve outbreaks by helping to quickly link clinical and retail product samples and assist in outbreak investigations. The PD (Dr. Thakur) laboratory, in partnership with the NC Department of Agriculture and Consumer Services (NCDACS), is a GenomeTrakr collaborating laboratory. Our proposal will significantly assist us in tracking emergence of potential new Salmonella, Listeria and STEC strains and allow the public health agencies to take appropriate steps to safeguard human and animal health at the global level. The specific objectives of our proposal are: 1) Establish a robust surveillance system based on whole genome sequence (WGS) profile of 400 Salmonella, Listeria and STEC isolated from food and environmental sources within and outside the US. 2) Compare the WGS profiles of Salmonella, Listeria and STEC at the global scale to assist in outbreak investigations and track emerging strains of public health importance. The long-term objective of our proposal is to protect and promote public health by strengthening the surveillance of Salmonella, Listeria and STEC within and outside the US by generating critical WGS data and contributing to the GenomeTrakr mission.