Core B

密西西比州围产期研究卓越中心 研究资源核心B 摘要 CEPR研究资源核心B的目标是向Cobre调查人员和受训人员提供 获得进行研究所需的最先进的设备和技术专长。新的子核 将在此Cobre中开发包括儿科超声心动图(ECHO)子核、遥测 子核和荧光激活细胞分类(FACS)子核。还将有生物统计学和 B核心中的生物信息学分核心，因为将有大量的基因组数据需要分析 对于项目IV，其他科布雷没有这方面的能力。这些子核心是新奇的，并不是 由现有的科布雷资助，心肾和代谢性疾病研究科布雷(P20 GM104357) (Cmdr)或精神病学神经科学中心(P30 GM103328)或南方大学 密西西比州INBRE项目。将向现有的Cmdr Cobre子核投入更多资源，供 CEPR调查人员、生物分析分核心、基因组学核心以及组织学和成像分部- 核心。 研究资源核心B的具体目标包括： 1)提供最高质量的尖端临床和基础研究服务。 2)提供一种可同时进行新生儿和胎儿超声检查的儿科超声心动图分芯。 3)为慢性血液测量提供一个位于中心、装备精良的遥测子核心 啮齿动物的血压、心率、活动度和体温。 4)提供装备良好的荧光激活细胞分选亚核，可用于分离 免疫细胞，如T细胞群、巨噬细胞等，分组进行分析和量化。 5)为项目和各分核心收集的数据提供生物统计和生物信息学分析。 6)对关键设备进行维护和监督，并提供培训、技术支持和 监督所有调查人员、教职员工和学生正确使用核心B设备。 7)为Cmdr基因组学分核心总监执行基因组学工作提供薪酬和支持 项目四的血缘关系分析。 8)为Cmdr液相色谱/质谱子核心提供薪金和支持 为项目II和项目III开发和测定性类固醇。 9)为CMDR生物分析亚核提供支持，以使CEPR Cobre 调查人员可以获得完成项目所需的尖端RIA和ELISA法检测 I-III。 10)为CMDR组织学和成像分核心提供供应支持，以便初级调查人员 获取最先进的成像技术(特别是计算机断层扫描)以及组织学和形态学。 11)优化所有化验和核心服务，改进现有化验和服务，并 协助解释结果和数据。 12)为Core B开发符合联邦资助指导方针的业务模式，以提供 支持核心B服务、设备和设施的可持续性。 核心B的服务对于成功完成 项目I-IV，将成为管道赠款调查人员的一项资产。此外，任何其他UMMC调查人员 将可以使用按存储容量使用计费的核心B服务。

MISSISSIPPI CENTER OF EXCELLENCE IN PERINATAL RESEARCH (CEPR) RESEARCH RESOURCES CORE B SUMMARY The goal of the Research Resources Core B of the CEPR is to provide COBRE investigators and trainees with access to state-of-the-art equipment and technical expertise needed to perform their research. New Sub-cores that will be developed in this COBRE include a Pediatric Echocardiography (ECHO) Sub-core, a Telemetry Sub-Core, and a Fluorescence-Activated Cell Sorting (FACS) Sub-Core. There will also be a Biostatistics and Bioinformatics Sub-Core in Core B since there will be considerable genomic data that will need to be analyzed for Project IV and there is no capacity for this in the other COBREs. These sub-cores are novel and are not funded by the existing COBREs, the Cardiorenal and Metabolic Diseases Research COBRE (P20 GM104357) (CMDR) or the Center for Psychiatric Neurosciences COBRE (P30 GM103328) or the University of Southern Mississippi INBRE program. Additional resources will be put into existing CMDR COBRE Sub-Cores for use by the CEPR investigators, the Bioanalytical Sub-Core, the Genomics Core and the Histology and Imaging Sub- Core. The Specific Aims of the Research Resources Core B include the following: 1) To provide services that foster cutting edge clinical and basic research of the highest quality. 2) To provide a Pediatric Echocardiography Sub-Core that can perform both neonatal and fetal sonography. 3) To provide a centrally located, well-equipped Telemetry Sub-Core for the chronic measurement of blood pressure, heart rate, activity and body temperature in rodents. 4) To provide a well equipped Fluorescence-Activated Cell Sorting Sub-Core that can be used to separate immune cells, such as populations of T cells, macrophages, etc, into groups for analysis and quantification. 5) To provide Biostatistics and bioinformatics analyses of data collected in the Projects and by the Sub-Cores. 6) To provide maintenance and supervision of key equipment and provide training, technical support, and supervision for all investigators, staff and students in the proper utilization of Core B equipment. 7) To provide salary and supply support for the CMDR Genomics Sub Core Director to perform genomic analyses for consanguinity for Project IV. 8) To provide salary and supply support for the CMDR Liquid Chromatography/Mass Spectroscopy Sub-Core to develop and measure sex steroids for Project II and III. 9) To provide supply support for the CMDR Bioanalytical Subcore so as to allow the CEPR COBRE investigators to have access to cutting edge RIA and ELISA assays necessary for completion of their Projects I-III. 10) To provide supply support for the CMDR Histology and Imaging Sub-Core so junior investigators have access to state-of-the-art imaging (specifically, computed tomography), and histology and morphology. 11) To optimize all assays and core services, to provide improvement in existing assays and services, and to assist in the interpretation of results and data. 12) To develop a business model for Core B that is compliant with federal funding guidelines to provide support for sustainability of Core B services, equipment, and facilities. The services of Core B are absolutely necessary for the successful completion of the studies described in Projects I-IV and will be an asset to Pipeline grant investigators. Furthermore, any other UMMC investigators will have access to the Core B services on a charge-back fee basis.