Core B - Resource for Pre-Clinical Studies of AKI

项目摘要 人类疾病的小动物模型为肾脏病理生理学提供了重要的见解， 过程，是一个重要的临床前资源，以测试治疗和预防方法，在急性 肾损伤（阿基）。核心B的具体目标是提供设施和必要的技能1）学习 阿基的鼠模型，2）用于小动物成像，和3）用于确定 阿基这一核心将具体提供（i）啮齿类动物模型开发和培训方面的专门知识， 阿基特别是在缺血/再灌注损伤、脓毒症和肾移植的情况下，（ii）多- 模态小动物成像核心，将提供最先进的分子成像，包括功能， 使用磁共振成像/光谱学的结构和代谢成像，高频 超声检查、microCT、伽马射线成像（伽马相机、microSPECT/CT、microPET/CT）和光学 成像（生物发光和荧光），以及（iii）生理学核心，将提供专业知识和培训 用于在整个肾脏和单个肾单位水平上研究肾功能，包括显微穿刺 肾小球滤过率测定技术，肾小管液微量分析和肾小管重吸收，肾 血流动力学与肾小管-肾小球反馈评估和肾氧代谢评估 啮齿动物的消费。核心B还将提供隔离一次油管和 啮齿类动物的血管细胞 核心B的目的是提供独特的资源，帮助克服研究者利用的障碍 用于体内研究的相关啮齿动物模型和用于体外研究的啮齿动物肾细胞和细胞系， 了解阿基的病理生理学。核心B在支持肾脏方面非常成功 研究社区。自奥布莱恩中心成立以来，核心B已经完成了12000多场演出 200名调查员。每年都有稳定数量的研究者使用核心B。本 在供资周期内，120名调查员中，91名（76%）是非核心调查员。核心B还支持 20个试点和可行性和催化剂奖得主的研究工作。这些共同努力 目前在105个同行评审出版物中得到认可。先进的基础设施加上 核心B的独特专业知识将继续促进我们研究者基础的合作活动， 阿基研究进展。

Project Summary Small animal models of human disease have provided important insights into renal pathophysiological processes and are an important preclinical resource to test therapeutic and preventive approaches in acute kidney injury (AKI). The specific aims of Core B are to provide the facilities and requisite skills 1) to study murine models of AKI, 2) for small animal imaging, and 3) to determine renal pathophysiological changes in AKI. This core will specifically provide (i) expertise in development and training in the use of rodent models of AKI specifically in the setting of ischemia/reperfusion injury, sepsis and kidney transplantation, (ii) a multi- modality small animal imaging core that will provide state-of-the-art molecular imaging, including functional, structural and metabolic imaging using magnetic resonance imaging/spectroscopy, high frequency ultrasonography, microCT, gamma-ray imaging (gamma camera, microSPECT/CT, microPET/CT), and optical imaging (bioluminescence and fluorescence), and (iii) a physiology core that will provide expertise and training for studying renal function on the whole kidney and at the single nephron level, including micropuncture techniques and determination of GFR, microanalysis of tubular fluid and tubular reabsorption, renal hemodynamics with assessment of tubuloglomerular feedback, and metabolic assessment of kidney oxygen consumption in rodents. Core B will also provide technical expertise for the isolation of primary tubular and vascular cells from rodents. The intent of Core B is to provide unique resources that help overcome barriers for investigators to utilize relevant rodent models for in vivo studies and rodent kidney cells and cell lines for in vitro studies to advance understanding of the pathophysiology of AKI. Core B has been very successful in supporting the kidney research community. Since the inception of the O'Brien Center, Core B has performed more than 12,000 procedures for 200 investigators. A steady number of investigators use Core B each year. In the present funding cycle, of the 120 investigators, 91 (76%) were non-core investigators. Core B has also supported the research efforts of 20 Pilot and Feasibility and Catalyst grant awardees. These combined efforts have been currently recognized in 105 peer-reviewed publications. The sophisticated infrastructure coupled with the unique expertise of Core B will continue to catalyze collaborative activities of our investigator base and advances in AKI research.