Einstein Mount Sinai Diabetes Research Center

动物生理学核心(APC)使用复杂的研究方法来帮助爱因斯坦-芒特 西奈糖尿病研究中心(ES-DRC)研究人员在体内评估葡萄糖和脂肪酸 小鼠和大鼠的代谢、胰岛素敏感性和能量稳态。通过与 作为ES-DRC的其他核心，APC使调查人员能够彻底表征定义的 药物、饮食、环境和遗传改变对糖脂平衡、胰岛素作用、 和新陈代谢。为了实现这些目标，动物生理学核心将：1)提供建议和指导 学生，博士后研究员，研究人员和技术人员在生理学的设计和表现 评价糖尿病患者血糖稳态控制和胰岛素作用的方法和技术 啮齿动物，2)向研究人员提供全身和组织特异性血糖的专门测量 包括胰岛素、胰腺和高血糖钳夹在内的啮齿动物模型的代谢和胰岛素作用 自发血糖监测，3)提供专门的胃肠道、神经外科和组织学模型 用于研究胰岛素敏感性、能量平衡、葡萄糖和脂肪酸代谢，包括胃 搭桥术与脂肪和肝组织失神经、成像和光刺激，4)提供整体分析 体内碳水化合物/脂肪酸氧化、能量消耗、产热、食物摄入量和运动活动 使用专门的代谢(间接量热法)啮齿动物笼子，5)提供饮食效果的评估， 运动、光/暗周期和环境温度对血糖的连续和急性评估 通过肌电图仪进行动态平衡、新陈代谢和颤抖，为专门的研究人员提供 用磁共振波谱、显微CT测量啮齿动物脂肪组织分布 作为肝脏和肌肉中糖原、肝内脂类和心肌细胞内脂类的核测量 磁共振(核磁共振)，7)为研究人员提供专门的大脑能量测量和 功能磁共振成像(FMRI)和微型PET扫描对葡萄糖的利用，辅助 研究人员解释数据并设计进一步的实验方法来揭示分子 和代谢相关的啮齿动物表型的生理基础，以及9)促进和整合 APC提供的功能评估与其他ES-DRC生物医学核心提供的分析。所有这些都是 糖尿病研究的新手以及从事糖尿病研究的研究人员都可以获得服务。 可通过利用该核心的专门知识和设施丰富和扩展的相关项目。

The Animal Physiology Core (APC) employs sophisticated research methodologies to assist Einstein-Mount Sinai Diabetes Research Center (ES-DRC) investigators in the in vivo assessment of glucose and fatty acid metabolism, insulin sensitivity and energy homeostasis in mice and rats. Through collaborative efforts with the other Cores of the ES-DRC, the APC enables investigators to thoroughly characterize the effects of defined pharmacologic, dietary, environmental and genetic alterations on glucose and lipid homeostasis, insulin action, and metabolism. To accomplish these goals, the Animal Physiology Core will: 1) Offer advice and instruction to students, postdoctoral fellows, investigators and technical staff in the design and performance of physiologic approaches and techniques necessary to evaluate the control of glucose homeostasis and insulin action in rodents, 2) Make available to investigators specialized measurements of whole body and tissue-specific glucose metabolism and insulin action in rodent models including insulin, pancreatic and hyperglycemic clamps and spontaneous glucose monitoring, 3) Provide specialized gastrointestinal, neurosurgical and histological models for the study of insulin sensitivity, energy balance, and glucose and fatty acid metabolism, including gastric bypass and adipose and hepatic tissue denervation, imaging and photo-stimulation, 4) Provide analysis of whole body carbohydrate/fatty acid oxidation, energy expenditure, thermogenesis, food intake, and locomotor activity using specialized metabolic (indirect calorimetry) rodent cages, 5) Provide assessments of the effects of diet, exercise, light/dark cycle and environmental temperature on continuous and acute assessments of glucose homeostasis, metabolism, and shivering via electromyography, 6) Make available to investigators specialized measurements of rodent adipose tissue distribution using magnetic resonance spectroscopy, microCT, as well as measurements of glycogen in liver and muscle, intrahepatic lipids and intramyocellular lipids using nuclear magnetic resonance (NMR), 7) Make available to investigators specialized measurements of brain energy and glucose utilization by functional magnetic resonance imaging (fMRI) and microPET scanning, 8) Assist investigators in the interpretation of data and to design further experimental approaches to reveal the molecular and physiological bases of metabolically relevant rodent phenotypes, and 9) Facilitate and integrate the functional assessments provided by the APC with assays provided by other ES-DRC Biomedical Cores. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes- related projects that can be enriched and extended by the use of the expertise and facilities of this Core.