Development of Sensitive and Specific Proteomic Biomarkers of Aging, Health, Frailty, and Morbidity in Human Cohorts

在我们之前开发衰老生物标志物特征的基础上，我们将重点放在发现单核细胞中发现的一组新的衰老生物标志物上，单核细胞是一种接近血液的细胞类型，已知随着人类年龄的增长衰老会增加。在过去的一年里，我们建立了衰老单核细胞培养，并对衰老的人类单核细胞进行了大规模的蛋白质组学分析。此外，为了测试这组细胞类型特异性生物标志物及其与人类衰老和疾病的关系，我们正在几个队列中启动研究：GESTALT、BLSA和InCHIANTI，以将一组新的生物标志物候选物与人类健康、虚弱和其他临床参数关联起来。

Building on our previous efforts to develop senescence biomarker signatures, we have focused our efforts on the discovery of a novel set of senescence biomarkers found in monocytes, a cell type that is both proximal to the blood and known to increase in senescence with age in humans. In the past year, we have established a senescent monocyte culture and performed large-scale proteomic analysis of senescent human monocytes. Further, to test this cell-type specific set of biomarkers and its relationship to aging and diseases in humans, we are initiating studies in several cohorts: GESTALT, BLSA, and InCHIANTI, to correlate a new set of biomarker candidates with human health, frailty, and other clinical parameters. The purpose and scope of this research project going forward is to develop and apply state-of-the-art mass spectrometry based proteomic techniques to discover and validate subsets of proteins and protein forms that are representative of aging and disease burdens in human tissues, blood, and other biofluids.