Development of Rapid, Miniaturized Sensors in Detection of Environmental Toxins

微型生物传感器可以使毒理学项目以高通量进行分析，并有可能具有更高的灵敏度。生物传感器项目旨在将毒理学项目中开发的那些生物检测方法转化为可用的生物传感器。该项目将有一个基本的方面，并在一个应用方面，我们打算实施这些分析。基础方面将研究用于生物的新型纳米材料。 标记，特别是在免疫分析中的应用。长寿命纳米荧光粉已被发现在免疫分析中标记半抗原、分析物或抗体特别有用。我们将侧重于镧系元素的使用，特别是Eu，以及通过使用Tb氧化物等材料可以提供的其他波长。我们还将研究一种在微滴中进行免疫分析的新形式。样本 我们的光漂白标签可以对含有微微升的物质进行长时间的检测，有可能在分析中接近单分子检测限值。该项目更实际的方面将涉及在芯片上的微型生物传感器中实施现有的分析。我们将利用微制造技术在芯片上制造微通道，在其中我们将进行免疫分析。我们将使用铟锡 氧化物(ITO)薄膜作为波导和电极来操纵通道中的纳米颗粒、标签和抗体。我们将使用通道内颗粒标记的消逝检测，并使用静电场来增强与抗体的结合，并潜在地在通道内再生抗体。我们还将尝试改进土壤中DNA的检测以进行采样。这将有助于项目1对土壤中的毒素消耗细菌进行测量。生物传感器项目将实施二恶英的体外检测，以供丹尼森博士的项目使用，并将与莱斯利博士的项目合作，实现一种微型便携式生物传感器，用于检测生殖健康的标志。

Miniaturized biosensors can enable the toxicology projects to undertake their assays with high throughput and potentially with greater sensitivity. The Biosensor project aims to implement those bio assays that have been developed in the toxicology projects into usable biosensors. The project will have a fundamental aspect, and an applied aspect in which we intend to implement these assays. The fundamental aspects will investigate new nano scale materials for bio labeling, particularly with application to immunoassays. Long lifetime nano scale phosphors have been found to be particularly useful for labeling haptens, analytes, or antibodies in an immunoassay. We will focus on the use of the lanthanide elements, in particular europium, and also other wavelengths that can be offered by the use of materials such as terbium oxide. We shall also investigate a novel format for carrying out immunoassays in a micro droplet. Samples that contain pico liters can be interrogated for very long times with our photobleaching labels, with the potential for approaching single molecule detection limits in assays. The more practical aspect of the project will be concerned with implementing existing assays in miniaturized biosensors on a chip. We shall make use of micro fabrication techniques to make micro channels on a chip in which we shall carry out the immunoassays. We shall make use of indium tin oxide (ITO) films as waveguides and as electrodes to manipulate nanoparticles labels and antibodies in channels. We shall use evanescent detection of the particle labels within the channel, and use an electrostatic field to enhance binding to antibodies, and potentially to regenerate antibodies within the channel. We shall also attempt to improve the detection of DNA for sampling in soils. This will assist Project 1 in undertaking their measurements of toxin-consuming bacteria within soils. The Biosensor project will implement an in vitro assay for dioxin for use in Dr. Denison's project and will work with Dr. Lasley's project to implement a miniaturized, portable biosensor for markers of reproductive health.