PROTEOMICS CORE

蛋白质组学核心的中心目标是提供蛋白质组学专业知识和最先进的 通过国家中心获得的蛋白质组学数据采集、挖掘和解释资源 用于项目I-III的蛋白质组学研究，以测试各个假设。每个项目都将利用 蛋白质组学核心的资源在其特定的目标，和蛋白质组学分析研究是至关重要的 每个项目的组成部分。蛋白质组学核心将与所有项目互动，以协助样本 蛋白质组学实验前和实验中的准备、质量控制以及分析和解释 的数据. MCW国家蛋白质组学研究中心的基础设施已经建立， 开发了优化的工具，非常适合蛋白质组的定量分析和表征， 蛋白质磷酸化的变化。这些工具、资源和专业知识将推动这三个项目的发展 并生成独特的数据集，突出麻醉剂的复杂潜在机制 预处理 对于这个PPG，蛋白质组学核心将 1)建立和改进质谱分析的标准分析程序， 细胞亚蛋白质组和蛋白质磷酸化的定量。 2)对线粒体和细胞溶质样品进行质谱分析， 项目I-III的调查人员。 3)使用同位素标记和串联质谱法定量蛋白质组学变化。 4)使用质谱方法分析蛋白质磷酸化的变化。 5)为所有三种蛋白质组样本提供可靠、可重复、统一和及时的分析 项目 6)维护数据库和自动化分析程序，用于蛋白质鉴定，定量， 路径分析。 7)提供专业知识和设备，协助调查人员进行数据分析和解释。

The central objective of the Proteomics Core is to provide proteomics expertise and state-of-the-art resources for proteomics data acquisition, mining, and interpretation available through the National Center for Proteomics Research for Projects l-lll to test individual hypotheses. Each individual project will utilize the resources of the Proteomics Core in their specific aims, and the proteomic profiling studies are critical components of each individual Project. The Proteomics Core will interact with all projects to assist in sample preparation, quality control before and during proteomic experiments, and with the analysis and interpretation of data. The established infrastructure of the National Center for Proteomics Research at MCW has developed optimized tools ideally suited for the quantitative analysis of proteomes and the characterization of changes in protein phosphorylation. These tools, resources, and expertise will advance all three projects effectively and generate unique datasets highlighting the complex underlying mechanisms of anesthetic preconditioning. For this PPG, the Proteomics Core will 1) Establish and improve standard analytical procedures for mass spectrometric analysis and quantification of cellular sub-proteomes and protein phosphorylation. 2) Perform mass spectrometric analyses of mitochondrial and cytosolic samples provided by the investigators of Projects l-lll. 3) Quantify proteomic changes using isotopic labeling and tandem mass spectrometry. 4) Analyze changes in protein phosphorylation using mass spectrometric approaches. 5) Provide reliable, reproducible, uniform and timely analyses of proteomic samples for all three projects. 6) Maintain databases and automated analysis procedures for protein identification, quantification, and pathway analysis. 7) Provide expertise and equipment to assist investigators in data analysis and interpretation.