Safeguarding Genetic Resources of Aquatic Biomedical Models

项目概要/摘要： 水产种质和遗传资源中心的活动有所扩大 在过去的5年里，我们正在为所有五个NIH资助的项目开发知识库。 水产养殖中心可靠的液氮供应对于大部分工作至关重要。巴吞鲁日 拥有强大的工业基础，液氮可从当地供应商获得。然而，交付和 现场管理已经变得越来越成问题，并且越来越成为我们研究的限制， 培训、外联、社区支持和样本储存活动。我们目前使用超过7000个 每年10加仑液氮，以约120个单独的230升低压滚动的形式输送 杜瓦我们每周都会收到并与供应商交换这些产品，但交付情况并不可靠 在预定时间到达时，坦克通常是空的或坏的。供应商还提供 高压罐会损坏我们的设备这要求AGGRC人员在以下情况下在场： 送货卡车到达，以确保事情做得很好，门被锁上时， 送货车开走了在飓风或其他破坏性事件之后，交付尤其不可靠。的 底线是，该中心已经发展到我们需要外部大容量存储（3，000 L）的程度 液氮罐，可以安装在现有的混凝土垫，这是为该目的而建造的。 此外，转向批量运输将使每升氮气成本降低28%。此外，我们的主要 样品处理仪器（Minitube，Quattro）用于填充、密封和标记冻存管， #21030;了10年，失去了可靠性。该设备还用于为以下产品贴标签： 储存中心和其他研究社区成员使用的低温保存吸管。我们需要 更换此设备以继续提供可靠的服务。例如，打印机单元已经被 更换为新型号，供应商将不再服务旧版本。替换将 减少现有仪器的使用，使我们能够延长其备用和溢流的工作寿命 操作因此，我们有很大的需要，以提高我们的液氮供应和使用，并取代 日常使用的必备设备。每一项改进都是对我们 作为满足社区需求的服务中心的增长，以及我们作为中心与所有人的互动增加 五个由NIH资助的国家水产品储备中心。如果得到资助，这一补充将大大改善 它将提供一个持续的强大的利益，以整体 研究社区。此外，这些改进将不需要额外的资金， 事实上，它们将大大降低我们的液氮成本。这项工作可以 在当前项目年度（进入第4年）内完成，并且与 或通过以前的补充资金。

Project Summary/Abstract: The activities at the Aquatic Germplasm and Genetic Resources Center (AGGRC) have expanded considerably over the past 5 years with us now addressing repository development for all five NIH-funded aquatic stock centers. Reliable availability of liquid nitrogen is essential to most of that work. Baton Rouge has a strong industrial base, and liquid nitrogen is available from local suppliers. However, delivery and on-site management have become increasingly problematic and are a growing constraint to our research, training, outreach, community support, and sample storage activities. We currently use more than 7,000 gallons of liquid nitrogen per year, delivered in the form of ~120 separate 230-liter low-pressure rolling dewars. We receive and exchange these with a vendor each week, but the deliveries cannot be relied upon to arrive when scheduled, and the tanks routinely arrive empty or broken. The vendors also deliver high-pressure tanks that can damage our equipment. This requires AGGRC personnel to be present when the delivery truck arrives to ensure things are done properly and that the doors are locked when the delivery truck leaves. Delivery is especially not reliable after hurricanes or other disruptive events. The bottom line is that the center has grown to the extent that we require an external bulk storage (3,000-L) liquid nitrogen tank that can be installed on an existing concrete pad that was built for that purpose. Moreover, switching to bulk delivery will reduce per-liter nitrogen cost by 28%. In addition, our primary sample processing instrument (Minitube, Quattro) that fills, seals, and labels cryopreservation straws is >10 years old and is losing reliability. This piece of equipment is also used to provide labeling of cryopreservation straws used by the stock centers and other research community members. We need to replace this equipment to continue to provide reliable services. For example, the printer unit has been replaced in newer models and the supplier will no longer service the old version. Replacement would reduce use of the existing instrument and allow us to extend its working lifetime for back-up and overflow operation. Thus, we have great need to upgrade our liquid nitrogen supply and use, and to replace an essential piece of equipment that is used daily. Each of these improvements is in direct response to our growth as a service center addressing community needs, and our increased interaction as a Hub with all five NIH-funded national aquatic stock centers. If funded, this supplement will substantially improve operations relative to the parent grant, and it will provide a sustained powerful benefit to the overall research community. In addition, these improvements will not require additional funding beyond the supplement, and they will in fact substantially reduce our costs for liquid nitrogen. This work can be completed within the current project year (entering Year 4), and it does not overlap with work funded in the parent award or through previous supplemental funding.