Safeguarding Genetic Resources of Aquatic Biomedical Models

保护水生生物医学模型遗传资源

• 批准号: 10600565
• 负责人: Maria Teresa Gutierrez-Wing
• 金额: $ 17.76万
• 依托单位: LOUISIANA STATE UNIV AGRICULTURAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-15 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10600565
• 关键词: Address; Adult; Algae; Ambystoma; Amphibia; Animal Model; Animals; Aplysia; Aplysia species; Award; Basic Science; Biocompatible Materials; Biological; Biological Models; Collaborations; Communities; Community Developments; Consensus; Cryopreservation; Databases; Development; Documentation; Education and Outreach; Educational Curriculum; Educational process of instructing; Educational workshop; Embryo; Engineering; Ensure; Evolution; Fertilization; Foundations; Freezing; Funding; Genetic; Genetic Models; Genetic Variation; Growth; Human Resources; Individual; Information Systems; Internet; Invertebrates; Kentucky; Knowledge; Laboratories; Larva; Life; Link; Location; Maintenance; Methods; Microfluidics; Modeling; Monoclonal Antibody R24; Oocytes; Organism; Parents; Pathway interactions; Population; Postdoctoral Fellow; Process; Production; Productivity; Protocols documentation; Rana; Recommendation; Recovery; Reproducibility; Research; Research Infrastructure; Resources; Risk; Salamander; Sampling; Schedule; Secure; Services; Shipping; Somatic Cell; Source; Standardization; System; Technology; Time; Training; Training Programs; Transgenic Organisms; United States National Institutes of Health; Universities; Wood material; Work; Xenopus; Xiphophorus; Zebrafish; aquatic organism; base; biosecurity; coronavirus disease; cost; egg; experimental study; extreme weather; genetic information; genetic resource; improved; innovation; mature animal; mutant; next generation; novel strategies; outreach; outreach program; preservation; programs; protocol development; repository; sperm cell; stem cells; success

Project Summary/Abstract: Aquatic organisms are powerful biomedical models used in laboratories around the world. These animals are maintained mostly as live organisms, with the associated expense and risk. As thousands of new lines of mutants and transgenic organisms are generated, maintaining populations of each becomes prohibitive for stock centers. To address these problems, cryopreservation has become a necessity for the preservation of genetic resources. Cryopreservation must be linked to development of germplasm repositories where genetic resources are protected in a reproducible, standardized way. A well developed and maintained repository can reduce the cost of maintaining and recovering lines, reduce duplication of effort, and reduce the costs required to recreate lines. We are working to develop appropriate processes and repository technology for amphibians (i.e., Ambystoma and Xenopus) and invertebrates (i.e., Aplysia). Our current parent award is intended to provide access to community-level approaches for reproducibility, training, and standardization through a centralized resource Hub and a repository network that can address aquatic biomedical model repository needs. Through R24 funding the Hub is establishing capabilities to process germplasm samples and develop pathways for repository development. Currently, animals used for the project are sent from stock centers and other laboratories to the AGGRC. Our reliance on outside sources has become a substantial problem in ensuring the availability of adequate numbers of animals for replicated studies and outreach activities. Supplemental funds are requested herein to enable us to spawn and grow animals in-house. This will greatly advance the objectives of the parent award by increasing research productivity and outreach capability. Also, the risks associated with animal shipping, particularly in extreme weather conditions will be diminished. This work can be completed within the current project year (entering Year 3), and it does not overlap with work funded in the parent award or through previous supplemental funding. The specific use of this request is to provide in-house production live amphibians cultured from spawning through adulthood, to provide a timely source of broodstock to support the activities of the parent award in research, teaching, training, collaboration, and outreach.

项目摘要/摘要： 水生生物是全球实验室中使用的强大生物医学模型。这些动物 主要是作为活生物体维持的，并具有相关的费用和风险。作为成千上万的新线条 产生突变体和转基因生物，维持每种生物的种群 用于股票中心。为了解决这些问题，冷冻保存已成为 保存遗传资源。冷冻保存必须与种质的发展有关 以可重复的，标准化的方式保护遗传资源的存储库。一个发达的 维护存储库可以降低维护和恢复线的成本，减少重复 努力，并降低重新创建线路所需的成本。我们正在努力开发适当的流程 以及两栖动物（即Ambystoma和Xenopus）和无脊椎动物（即Aplysia）的存储库技术。 我们目前的父母奖项旨在为社区级别的方法提供访问权限 通过集中资源中心和存储库网络的可重复性，培训和标准化 这可以满足水生生物医学模型存储库的需求。通过R24资金，枢纽正在建立 处理种质样品并开发存储库开发的途径的能力。现在， 用于该项目的动物是从股票中心和其他实验室发送到AGGRC的。我们的依赖 外部来源已成为确保足够数量的可用性的重大问题 动物进行复制研究和外展活动。本文要求补充资金以启用 我们要在内部产卵和种植动物。这将大大提高父母奖的目标 提高研究生产力和外展能力。此外，与动物运输相关的风险， 特别是在极端天气条件下会减少。这项工作可以在 当前项目年度（进入第3年），并且与父母奖励中资助或 通过以前的补充资金。该请求的特定用途是提供内部生产 从产卵到成年的活着的两栖动物，为及时的繁殖来源提供给 在研究，教学，培训，协作和外展方面支持父母奖的活动。