Safeguarding Genetic Resources of Aquatic Biomedical Models

项目概要/摘要： 水生生物是世界各地实验室使用的强大生物医学模型。这些动物 大部分作为活的有机体维持，伴随着相关的费用和风险。成千上万的新线路 的突变体和转基因生物产生，保持每一个群体变得令人望而却步 库存中心。为了解决这些问题，冷冻保存已经成为生物医学的必需品。 保护遗传资源。超低温保存必须与种质开发相结合 以可复制、标准化的方式保护遗传资源的储存库。一个发展良好 和维护的存储库可以降低维护和恢复线路的成本，减少重复， 努力，并降低重新创建线路所需的成本。我们正在努力制定适当的程序， 和两栖动物的储藏技术（即，Ambystoma和Xenopus）和无脊椎动物（即，A.A.）。 我们目前的家长奖旨在提供社区一级的方法， 通过集中资源中心和知识库网络实现可重复性、培训和标准化 可以满足水生生物医学模型储存库的需求。通过R24资金，该中心正在建立 有能力处理种质样本，并为储藏库的发展开辟道路。目前， 用于该项目的动物从储备中心和其他实验室送到AGGRC。的依赖 在确保提供足够数量的 用于重复研究和推广活动的动物。在此请求补充资金， 让我们在家里产卵和养殖动物。这将大大推进家长奖的目标， 提高研究生产力和推广能力。此外，与动物运输相关的风险， 特别是在极端天气条件下，将减少。这项工作可以在 当前项目年度（进入第3年），并且它不与父奖项资助的工作重叠，或者 通过此前的补充资金。这个请求的具体用途是提供内部生产 从产卵期到成年期养殖的活两栖动物，为繁殖提供及时的来源， 支持家长奖在研究、教学、培训、合作和推广方面的活动。

Project Summary/Abstract: Aquatic organisms are powerful biomedical models used in laboratories around the world. These animals are maintained mostly as live organisms, with the associated expense and risk. As thousands of new lines of mutants and transgenic organisms are generated, maintaining populations of each becomes prohibitive for stock centers. To address these problems, cryopreservation has become a necessity for the preservation of genetic resources. Cryopreservation must be linked to development of germplasm repositories where genetic resources are protected in a reproducible, standardized way. A well developed and maintained repository can reduce the cost of maintaining and recovering lines, reduce duplication of effort, and reduce the costs required to recreate lines. We are working to develop appropriate processes and repository technology for amphibians (i.e., Ambystoma and Xenopus) and invertebrates (i.e., Aplysia). Our current parent award is intended to provide access to community-level approaches for reproducibility, training, and standardization through a centralized resource Hub and a repository network that can address aquatic biomedical model repository needs. Through R24 funding the Hub is establishing capabilities to process germplasm samples and develop pathways for repository development. Currently, animals used for the project are sent from stock centers and other laboratories to the AGGRC. Our reliance on outside sources has become a substantial problem in ensuring the availability of adequate numbers of animals for replicated studies and outreach activities. Supplemental funds are requested herein to enable us to spawn and grow animals in-house. This will greatly advance the objectives of the parent award by increasing research productivity and outreach capability. Also, the risks associated with animal shipping, particularly in extreme weather conditions will be diminished. This work can be completed within the current project year (entering Year 3), and it does not overlap with work funded in the parent award or through previous supplemental funding. The specific use of this request is to provide in-house production live amphibians cultured from spawning through adulthood, to provide a timely source of broodstock to support the activities of the parent award in research, teaching, training, collaboration, and outreach.