Safeguarding Genetic Resources of Aquatic Biomedical Models

项目摘要/摘要： 水生生物是世界各地实验室中使用的强大的生物医学模型。这些动物 主要作为活的有机体维持，伴随着相关的费用和风险。数以千计的新线 产生了突变体和转基因生物，维持每种生物的种群就变得令人望而却步 为股票中心服务。为了解决这些问题，冷冻保存已经成为一种必要的手段 保存遗传资源。超低温保存必须与种质的发展相联系 以可复制、标准化的方式保护遗传资源的储存库。一个很发达的国家 和维护存储库可以降低维护和恢复生产线的成本，减少重复 努力，并降低重建线路所需的成本。我们正在努力开发适当的流程 以及两栖动物(即非洲爪哇和非洲爪哇)和无脊椎动物(即海兔)的储存库技术。 我们目前的家长奖旨在为以下项目提供社区级别的方法 通过集中的资源中心和存储库网络实现可重复性、培训和标准化 这可以满足水生生物医学模型库的需求。通过R24资金，该中心正在建立 处理种质样本和开发资源库开发途径的能力。目前， 用于该项目的动物被从饲养中心和其他实验室送到AGGRC。我们的依赖 关于外部来源已经成为确保获得足够数量的 用于重复研究和推广活动的动物。在此请求补充资金，以实现 我们在家中产卵和饲养动物。这将极大地推进父级奖项的目标 提高研究效率和推广能力。此外，与动物运输相关的风险， 特别是在极端天气条件下，将会减少。这项工作可以在 当前项目年度(进入第3年)，并且不与父奖项资助的工作或 通过以前的补充资金。此请求的具体用途是提供内部生产 从产卵到成体的活体两栖动物养殖，为亲鱼提供及时的来源 支持家长奖在研究、教学、培训、协作和推广方面的活动。

Project Summary/Abstract: Aquatic organisms are powerful biomedical models used in laboratories around the world. These animals are maintained mostly as live organisms, with the associated expense and risk. As thousands of new lines of mutants and transgenic organisms are generated, maintaining populations of each becomes prohibitive for stock centers. To address these problems, cryopreservation has become a necessity for the preservation of genetic resources. Cryopreservation must be linked to development of germplasm repositories where genetic resources are protected in a reproducible, standardized way. A well developed and maintained repository can reduce the cost of maintaining and recovering lines, reduce duplication of effort, and reduce the costs required to recreate lines. We are working to develop appropriate processes and repository technology for amphibians (i.e., Ambystoma and Xenopus) and invertebrates (i.e., Aplysia). Our current parent award is intended to provide access to community-level approaches for reproducibility, training, and standardization through a centralized resource Hub and a repository network that can address aquatic biomedical model repository needs. Through R24 funding the Hub is establishing capabilities to process germplasm samples and develop pathways for repository development. Currently, animals used for the project are sent from stock centers and other laboratories to the AGGRC. Our reliance on outside sources has become a substantial problem in ensuring the availability of adequate numbers of animals for replicated studies and outreach activities. Supplemental funds are requested herein to enable us to spawn and grow animals in-house. This will greatly advance the objectives of the parent award by increasing research productivity and outreach capability. Also, the risks associated with animal shipping, particularly in extreme weather conditions will be diminished. This work can be completed within the current project year (entering Year 3), and it does not overlap with work funded in the parent award or through previous supplemental funding. The specific use of this request is to provide in-house production live amphibians cultured from spawning through adulthood, to provide a timely source of broodstock to support the activities of the parent award in research, teaching, training, collaboration, and outreach.