Peptide Core

蛋白质化学和多肽合成一直是该计划活动的一个突出特点 自1968年成立以来。最初，这些是作为组件项目的一部分执行的，该组件项目旨在 甲状旁腺激素和降钙素的结构分析及构效关系研究。此外 最早的活性合成钙调节多肽，对测序的基础和持久贡献 从这些调查中产生了合成方法论。这些化学品的用途越来越广泛 方法为所有项目带来了稳步扩大的好处；为了满足由此产生的需求，单独的 CORE成立于1979年。除了不断开发合成甲状旁腺素激动剂和 拮抗剂，核心继续发挥不同的作用，如识别体内甲状旁腺素代谢的产物， 一系列生物和免疫分析、表征和微测序的校准标准 重组表达产物和生物合成标记蛋白及其膜蛋白的特性 与成功纯化PTH/PTHrP受体的努力相结合。 随着内分泌科在20世纪80年代中期搬到威尔曼大楼(现在是他们的)实验室，S 在资源共享进程中迈出了第一步，这一进程至今仍在继续，为双方带来了重大好处 该计划和美国国立卫生研究院。1993年正式合并为一个共同的分析-综合设施， 分子内分泌学和生殖内分泌单位，紧随其后的伙伴艾滋病研究 中心，核心获得了重要的新能力，包括气相测序，微孔高效液相色谱和 增强了合成能力。当时和现在一样，运营成本是在个人之间仔细分配的 项目和调查人员根据利用率，但通过扩大对 方法、经验深度、人员部署的灵活性和规模经济是显而易见的 在上一次计划项目续订时明显。

Protein chemistry and peptide synthesis have been a prominent feature of the Program's activities since its inception in 1968. Originally, these were carried out as a part of a component Project aimed at the structural analysis and study of the structure-activity relations of parathyroid hormone and calcitonin. Besides the earliest active synthetic calcium-regulating peptides, fundamental and enduring contributions to sequencing and synthetic methodologies emerged from these investigations. The increasing versatility of these chemical methods brought steadily expanding benefits to all of the projects; to meet the resulting needs, a separate Core was established in 1979. In addition to continued development of synthetic PTH agonists and antagonists, the Core proceeded to fulfill such diverse roles as identifying products of in vivo PTH metabolism, calibrating standards for a range of biological and immunological assays, characterizing and microsequencing recombinant expression products and biosynthetically labeled proteins, and characterizing membrane proteins in conjunction with the successful effort to purify the PTH/PTHrP receptor. With the move of the Endocrine Unit into the Wellman (now Their) Building laboratory in the mid-1980's came the first step in the process of resource sharing that continues to this day to bring major benefits to both the Program and the NIH. By formally consolidating in 1993 into a common analytical-synthesis facility with the Molecular Endocrinology and Reproductive Endocrine Units, followed by the Partners AIDS Research Center, the Core gained important new capabilities including gas-phase sequencing, microbore HPLC and enhanced synthesis capacity. Then, as now, operating costs were carefully allocated among the individual projects and investigators according to utilization, but the benefits realized from widened access to methodology, depth of experience, flexibility in personnel deployment and economies of scale were clearly apparent at the time of the last Program Project renewal.