CORE--Peptide Core

CORE--肽核心

• 批准号: 6744655
• 负责人: HENRY T KEUTMANN
• 金额: $ 18.56万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6744655
• 关键词: biomedical facility; high performance liquid chromatography; mass spectrometry; parathyroid hormones; peptide chemical synthesis; peptides; protein purification; protein sequence

The Peptide Core provides the Program Project with a highly-responsive, in-house resource for peptide synthesis and chemical-analytical services and expertise through the following activities: 1. Preparation of synthetic peptides and analogs over a range of scales, followed by documentation of their homogeneity; 2. Performance of sequencing, mass spectroscopy, and amino acid compositional analyses of peptides and biosynthetic products; 3. Development and application of specialized chemical procedures such as crosslinking, prosthetic group attachment and "customized" purification modalities; 4. Micro- and macro-scale purification of proteins and peptides using HPLC and other chromatographic modalities 5. Advice and consultation in synthesis and purification strategies and "hands-on" transfer of technologies to participating investigators.

肽核心通过以下活动为计划项目提供高度响应的肽合成和化学分析服务和专业知识的内部资源： 1.在一定规模范围内制备合成肽和类似物，然后记录其均一性; 2.肽和生物合成产物的测序、质谱和氨基酸组成分析; 3.开发和应用专门的化学程序，如交联、辅基连接和“定制”纯化方式; 4.使用HPLC和其他色谱模式对蛋白质和肽进行微观和宏观纯化 5.在合成和纯化战略以及“动手”转让 参与研究者的技术。