CORE--PEPTIDE AND OLIGONUCLEOTIDE LABORATORY

核心--肽及寡核苷酸实验室

• 批准号: 6300959
• 负责人: HENRY T KEUTMANN
• 金额: $ 22.9万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6300959
• 关键词: biomedical facility; mass spectrometry; nucleic acid chemical synthesis; nucleic acid probes; oligonucleotides; parathyroid hormones; peptide chemical synthesis; protein purification; protein sequence

Description:(Taken directly from the application) The Peptide/ Oligonucleotide Core provides the Program Project with highly responsive, in-house resource for chemical-analytical and synthesis services and expertise through the following activities: (1) Preparation of synthetic peptides and analogs over a range of scales, followed by documentation of their homogeneity; (2) Performance of sequencing, mass spectroscopy, and amino acid compositional analyses of peptides and biosynthetic products; (3) Preparation of synthetic oligonucleotides for use as probes for screening, hybridization and site-directed mutagenesis; (4) Development and application of specialized chemical procedures such as cross linking, prosthetic group attachment and "customized" purification modalities; (5) Advice and consultation in synthesis and purification strategies and "hands-on" transfer of technologies to participating investigators.

描述：(直接取自申请)肽/寡核苷酸核心通过以下活动为计划项目提供高响应性的内部资源，用于化学分析和合成服务和专业知识：(1)准备各种规模的合成肽和类似物，然后记录它们的同质性；(2)多肽和生物合成产品的测序、质谱学和氨基酸组成分析的性能；(3)合成寡核苷酸的制备，用作筛选、杂交和定点突变的探针；(4)开发和应用专门的化学程序，如交联法、假体基团附着法和“定制”纯化方式；(5)在合成和纯化战略方面提供咨询和咨询，并向参与调查人员“亲手”转让技术。