Resource for Integrated Glycotechnology

综合糖技术资源的主要目标是多学科的发展， 解决糖生物学问题的方法。一个特定的焦点是与以下方面相关的问题的子集： 糖胺聚糖功能糖胺聚糖，如肝素、硫酸乙酰肝素和硫酸软骨素 在调节细胞内信号传导，影响免疫细胞向免疫细胞的位点的迁移中起重要作用。 感染，控制肿瘤中的血管生成，以及调节神经元的再生。它们也是 病原体的受体。为了实现这些作用，结合蛋白与细胞的特定区域相互作用。 这些糖胺聚糖。了解这些相互作用是干预的重要一步， 人类疾病，但很少有信息可用于识别的特定序列，结构 相互作用的方面，或者相互作用导致细胞反应的方式。缺乏 信息的部分原因是这些碳水化合物的序列非常复杂， 聚合物该资源开发技术，通过结合以下方面的进展来提供这一信息： 显示结合特异性的糖胺聚糖寡聚体的分离和合成， 核磁共振（NMR）中基于质谱（MS）的鉴定低聚物结构的方法 基于光谱和质谱的定义复合物三维结构的方法， 糖胺聚糖-蛋白质相互作用的计算建模和预测，以及在细胞和 基于生物化学的手段监测生物反应。技术的发展是由 与外部合作者一起选择推动生物医学项目。其中包括解决 Robo-Slit信号传导在血管生成中的功能，DLB结构域在疟疾患者存活中的功能， 胎盘寄生虫感染，特异性糖胺聚糖结合抗体检测细胞 异常，以及通过趋化因子调节免疫细胞迁移。技术传播 通过广泛的培训计划和额外的合作和服务职能主办的 Resource.

The primary objective ofthe Resource for Integrated Glycotechnology is the development of multidisciplinary approaches to the solution of problems in glycobiology. A particular focus is a subset of problems related to glycosaminoglycan function. Glycosaminoglycans such as heparin, heparan sulfate, and chondroitin sulfate play important roles in modulating intracellular signaling, influencing the migration of immune cells to sites of infection, controlling angiogenesis in tumors, and regulating regeneration of neurons. They also serve as receptors for pathogenic organisms. To fulfill these roles, binding proteins interact with specific regions of these glycosaminoglycans. Understanding these interactions is an important step toward intervention in human disease, yet little information is available on the specific sequences recognized, the structural aspects of the interactions, or they way in which interactions result in cellular response. The lack of information is in part due to the extraordinary complexity of the sequences of these carbohydrate based polymers. The Resource develops technology to provide this information by combining advances in separation and synthesis of glycosaminoglycan oligomers that display binding specificity, in mass spectrometry (MS) based means of identifying oligomer structures, in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry based means of defining three dimensional structures of complexes, in computational modeling and prediction of glycosaminoglycan-protein interactions, and in cell and biochemically based means of monitoring a biological response. The technology development is driven by selected driving biomedical projects with external collaborators. These include ones that address the function of Robo-Slit signaling in angiogenesis, the function of DLB domains in survival of the malaria parasite in placental infection, the specificity of glycosaminoglycan binding antibodies in detection of cellular abnormalities, and the regulation of immune cell migration by chemokines. Technology is disseminated through extensive training programs and additional collaborations and service functions hosted by the Resource.