Cell Imaging Shared Resource

细胞成像共享资源(CISR)：项目摘要/摘要 范德比尔特糖尿病研究和培训中心(VDRTC)将继续支持VDRTC细胞 成像核心，由细胞成像共享资源(CISR)运行，并确保VDRTC附属 调查人员将完全可以使用最先进的成像设备和专家技术支持来进行 对组织、细胞解剖学和生理学进行精密的显微镜和分析。通过利用一个大的，井- 成熟的设施，VDRTC细胞成像核心提供高水平的质量控制，专门的专家 对个人来说很难(如果不是不可能)的服务，以及显著的规模经济 调查人员要建立和支持。这一组织结构与集中化的方法非常契合 防止联邦资助的研究中心内不必要的科学资源重复，使重点 开发服务于范德比尔特所有研究的技术，包括VDRTC调查人员。整体而言 VDRTC细胞成像核心的目标是提供全方位的现代显微镜和数字成像 通过三个目标支持和加速范德比尔特糖尿病研究的能力和技术： 1)获取并保持最先进的光学和电磁成像技术；2)培训、协助和鼓励 VDRTC附属调查人员将光学、EM和活体成像技术纳入他们的研究； 以及3)开发对糖尿病研究有用的新的成像技术。如果没有VDRTC支持， 先进显微镜服务的广度和质量将受到严重限制。VDRTC支持补助金 还降低了向VDRTC调查人员提供成像服务的净成本，并帮助提供了财务稳定 对于维持对VDRTC研究人员的历史高水平服务至关重要。这种稳定对 成功收购支持VDRTC研究人员的新仪器和服务。在过去5年内 年，代表84个独立的VDRTC附属实验室的调查人员(比前一年增加了31% 循环)使用了《销售公约》的所有方面。在同一时期，《销售公约》为获得设备采取了主动行动 赠款加上机构捐款，提供了4 766 063美元的新资本设备，经管理 由CSR为VDRTC调查人员提供的。这包括超分辨率、双光子激发 荧光、多激发TIRF等先进显微镜。范德比尔特共享设施系统 为细胞成像核心提供高效的计费系统、监督和治理。VDRTC信元 成像核心将继续是范德比尔特公司广泛的糖尿病研究的关键组成部分 资源，并将提供基本服务，以支持VDRTC附属调查人员的研究 下一个资金周期。

Cell Imaging Shared Resource (CISR): Project Summary/Abstract The Vanderbilt Diabetes Research and Training Center (VDRTC) will continue to support the VDRTC Cell Imaging Core, operated by the Cell Imaging Shared Resource (CISR), and ensure that VDRTC-affiliated investigators will have full access to state-of-the-art imaging equipment and expert technical support to conduct sophisticated microscopy and analysis of tissue and cellular anatomy and physiology. By utilizing a large, well- established facility, the VDRTC Cell Imaging Core provides high levels of quality control, a dedicated, expert staff and a significant economy of scale for services that would be difficult (if not impossible) for individual investigators to establish and support. This organizational structure fits well with the centralized approach to prevent needless duplication of scientific resources within federally funded research centers, enabling focused development of technology that serves all Vanderbilt research, including VDRTC investigators. The overall goal of the VDRTC Cell Imaging Core is to provide the full range of modern microscopy and digital imaging capabilities and techniques to enable and accelerate diabetes research at Vanderbilt through three objectives: 1) acquire and maintain state-of-the art optical and EM imaging technology; 2) train, assist, and encourage VDRTC-affiliated investigators to incorporate optical, EM, and in vivo imaging technologies into their research; and 3) develop new imaging technologies that will be useful for diabetes research. Without VDRTC support, the breadth and quality of advanced microscopy service would be severely limited. The VDRTC support grant also reduces the net cost of imaging service to VDRTC investigators and has helped provide financial stability essential to sustain historically high levels of service to VDRTC researchers. This stability has been vital to the successful acquisition of new instruments and services that support VDRTC researchers. During the past 5 years, investigators representing 84 separate VDRTC-affiliated laboratories (a 31% increase from the prior cycle) have used all aspects of the CISR. Over the same period, CISR initiatives taken to obtain equipment grants, combined with institutional contributions, have provided $4,766,063 in new capital equipment, managed by the CISR, for the benefit of VDRTC investigators. This includes super-resolution, 2-photon-excited fluorescence, multi-excitation TIRF, and other advanced microscopies. The Vanderbilt shared facilities system provides an efficient billing system, oversight and governance for the Cell Imaging Core. The VDRTC Cell Imaging Core will continue to be a critical component in Vanderbilt's broad range of diabetes research resources and will provide essential services that support the research of VDRTC-affiliated investigators during the next funding cycle.