Susceptibility determinants to Legionella pneumophila infection in smokers

吸烟者嗜肺军团菌感染的易感性决定因素 摘要 军团菌是饮用水相关肺炎暴发的主要原因， 死亡。在免疫功能低下的老年人中，军团菌感染的风险更高，预后更差 还有吸烟者。一些流行病学研究表明，烟草和烟草之间存在很强的联系。 吸烟和军团菌感染风险增加。然而，潜在的机制仍不清楚。至 确定吸烟导致军团菌感染的机制，我们将进行以下工作 目标： 目的1.测定吸烟者肺泡巨噬细胞的甲基化组和RNAseq谱 (&gt；10包)和非吸烟者。目的2.确定甲基化改变的基因的功能和 表达并确定巨噬细胞对军团菌敏感性增加的原因 感染。

Susceptibility determinants to Legionella pneumophila infection in smokers Abstract Legionella accounts for majority of drinking water-associated pneumonia outbreaks with high percentage of death. Legionella infection presents with a higher risk and worse outcome in the elderly, immune-compromised and smokers. Several epidemiological studies have demonstrated a strong association between tobacco smoking and an elevated risk of Legionella infection. However, the underlying mechanism is still unclear. To determine the mechanism by which smoking predisposes to Legionella infection, we will perform the following aims: Aim 1. Determine the methylomic and RNAseq profile of human alveolar macrophages from current smokers (>10 pack years) and non-smokers. Aim 2. Determine the function of genes with altered methylation and expression and identify those responsible for the increased susceptibility of macrophages to Legionella infection.