Vanderbilt University Biomolecular Multimodal Imaging Center for 3-Dimensional Tissue Mapping

项目摘要 我们建议在范德比尔特建立生物分子多模式成像中心（BIOMIC 作为与人类生物分子图谱计划（HuBMAP）相关的组织图谱中心。 BIOMIC的使命是建立一个成像和分子分析集成技术平台， 能够构建人体组织的全面三维分子图谱。BIOMIC将 利用质谱研究中心和国家研究中心的独特资源， 范德比尔特大学沿着的成像质谱资源以及世界一流的临床 环境的范德比尔特大学医学中心，以创造一种能力，以表征人体组织在 这是当前技术无法比拟的理解水平。BIOMIC的创新之处在于 三维成像质谱（IMS）与各种体内和离体成像的组合 技术，以创造新的模式，分子知情的医学成像。BIOMIC的应用 肾脏、胰腺和骨骼中器官特异性项目的平台将提供一种新的理解范式 这些器官在多个维度上的正常状态，包括分子（例如，脂质、代谢物和 蛋白质）和空间（例如，整个器官到单个细胞）。作为HuBMAP的参与者， 由BIOMIC生成的数据将分发给世界各地的合作者，以促进生成和测试 关于这些重要器官系统功能的新假设，使人们对人类的新认识成为可能。 健康和疾病。BIOMIC的使命是创建一个平台，可以有效地获取和整合 多模态成像数据集，包括IMS、组织学、多重IHC、磁共振成像和高 高分辨率CT成像，用于形成全面的3-D分子图谱。这项工作需要 创建和整合三种能力，1）采购和管理标本，2）制备和缓解 预分析因素，以及3）获取、处理和传播多模态成像和大规模组学 数据BIOMIC平台将使用三个器官特异性项目进行优化和测试：骨、肾和 胰腺

Project Summary We propose the founding of the BIOmolecular Multimodal Imaging Center (BIOMIC) at Vanderbilt University as a Tissue Mapping Center associated with the Human BioMolecular Atlas Program (HuBMAP). The mission of BIOMIC is to build a platform of integrated technologies for imaging and molecular analysis that enables the construction of comprehensive 3-dimensional molecular atlases of human tissues. BIOMIC will leverage the unique resources of the Mass Spectrometry Research Center and the National Research Resource for Imaging Mass Spectrometry at Vanderbilt University along with the world-class clinical environment of the Vanderbilt University Medical Center to create a capability to characterize human tissues at a level of understanding unrivaled by current technologies. The innovative aspect of BIOMIC is the combination of 3-dimesional imaging mass spectrometry (IMS) with a variety of in vivo and ex vivo imaging technologies to create new modalities of molecularly-informed medical imaging. The application of the BIOMIC platform to organ-specific projects in kidney, pancreas, and bone will provide a new paradigm of understanding the normal state of these organs across multiple dimensions, both molecular (e.g., lipids, metabolites, and proteins) and spatial (e.g., whole organs to single cells). As a HuBMAP participant, the molecular atlases generated by BIOMIC will be disseminated to collaborators worldwide to facilitate the generation and testing of new hypotheses regarding the function of these important organ systems, enabling new insight into human health and disease. The mission of BIOMIC is to create a platform that can efficiently acquire and integrate multimodal imaging data sets, including IMS, histology, multiplex-IHC, magnetic resonance imaging, and high- resolution CT imaging for the formation of comprehensive 3-D molecular atlases. This effort requires the creation and integration of three capabilities to 1) procure and manage specimens, 2) prepare and mitigate pre-analytical factors, and 3) acquire, process, and disseminate multimodal imaging and large-scale omics data. The BIOMIC platform will be optimized and tested using three organ-specific projects: bone, kidney, and pancreas.