A High-Throughput Molecular Profiling Core for the CaST Center

项目摘要 分子分析核心将提供综合药物筛选和高通量表达谱分析， 单细胞RNA-Seq，以及基本的RNA-Seq和外显子组测序服务，为中心的每个项目提供服务。使用 我们最近开发的PLATE-Seq技术，我们可以产生高维表达谱， 高通量药物筛选中的每一个孔。我们还将提供PLATE-Seq的微流体实现 用于大规模单细胞表达谱分析，中心研究者将使用该分析来鉴定表型 肿瘤细胞的亚群会产生耐药性。中心研究人员还需要基本的 测序服务，包括深度外显子组和RNA-Seq，以确定突变， 单细胞水平和基因融合。核心将提供量身定制的和具有成本效益的图书馆准备， 为这些努力提供服务。

PROJECT SUMMARY The Molecular Profiling Core will provide integrated drug screening and high-throughput expression profiling, single cell RNA-Seq, and basic RNA-Seq and exome sequencing services to every project in the center. Using our recently developed PLATE-Seq technology, we can produce high-dimensional expression profiles across every well in a high-throughput drug screen. We will also provide a microfluidic implementation of PLATE-Seq for large-scale single cell expression profiling which will be used by center investigators to identify phenotypic subpopulations of tumor cells that give rise to drug resistance. Center investigators also require basic sequencing services including deep exome and RNA-Seq to identify mutations for targeted profiling at the single cell level and gene fusions. The Core will provide tailored and cost effective library preparation and sequencing services to facilitate these efforts.