Histology and Histomorphometry Core

该计划项目的四个项目严重依赖于使用鼠标模型来演示其 假设。因此，组织学和组织形态计量学核心的目的是巩固设备 和专业知识，为这些项目的研究人员提供最先进的、质量控制的组织学 骨、胰腺和脑标本的组织形态计量学分析以及小鼠骨骼的显微CT。 对此计划项目的核心贡献将在4个级别进行： 1.对小鼠标本进行组织学、免疫标记和组织形态计量学的处理。固定的和部分的 脱水的小鼠骨骼或胰腺将被嵌入塑料介质(脊椎和长骨)中 或石蜡(胰腺)。固定的小鼠脑标本要么不包埋，要么冷冻保存。 内嵌和冰冻切片。 2.对小鼠组织切片进行特异性染色和免疫染色。经典组织学染色 如苏木素/曙红、甲苯胺蓝、冯·科萨/凡·吉森、Goldner三色、甲酚紫等 以及更具体的反应，如TUNEL和陷阱染色，将由核心进行 人员应调查人员的要求。核心还将进行免疫检测研究，使用 切片上的荧光和酶检测。 3.对小鼠骨、胰腺和脑标本进行组织形态计量学分析。这个 将使用OsteoMeasure组织形态测量系统(OsteoMetrics Inc.，Decatur，GA)来评估静态 骨体积、骨小梁数量和细胞计数等参数，以及骨的动力学参数 从未脱钙的骨切片中形成矿物质，如矿物质沉积率。它还将被用来 量化胰腺细胞质量/增殖和脑切片中TUNEL或BrdU阳性细胞。图像J将 用于所有其他量化。核心将为调查人员提供组织形态测量数据和 将帮助它们评估它们的生物学相关性。 4.对小鼠骨标本进行显微CT分析。固定和部分脱水的小鼠长骨和 脊椎骨将由项目调查人员提供给Core。然后这些标本将被扫描 使用VivaCT 40微型CT(Sanco Medical，瑞士)。

The four projects of this Program Project heavily rely on the use of mouse models to demonstrate their hypotheses. The purpose of the Histology and Histomorphometry Core is therefore to consolidate equipment and expertise to provide the Investigators of these projects with state-of-the-art, quality controlled histologic and histomorphometric analyses of bone, pancreas and brain specimen as well as microCT of mouse bones. The Core contribution to this Program Project will be at 4 levels: 1. Processing mouse specimens for histology, immunolabeling and histomorphometry. Fixed and partially dehydrated mouse skeletons or pancreata will be embedded in plastic medium (vertebrae and long bones) or paraffin (pancreas). Fixed mouse brain specimen will either be sectioned without embedding or cryo- embedded and cryo-sectioned. 2. Performing specific stainings and immunostainings of mouse tissues sections. Classic histology stainings such as Hematoxylin/Eosin, Toluidine Blue, von Kossa/Van Gieson, Goldner trichrome, Cresyl violet as well as more specific reactive ones such as TUNEL and TRAP stainings will be performed by the Core personnel at the request of investigators. The Core will also perform immunodetection studies using fluorescent and enzymatic detection on sections. 3. Performing histomorphometric analyses of mouse bone, pancreas and brain specimens. The OsteoMeasure Histomorphometry System (OsteoMetrics Inc., Decatur, GA) will be used to assess static parameters, such as bone volume, trabecular number and cell counts, and dynamic parameters of bone formation, such as mineral apposition rates, from non-decalcified bone sections. It will also be used to quantify cell mass/proliferation in pancreas and TUNEL or BrdU positive cells in brain sections. Image J will be used for all other quantifications. The Core will provide investigators with histomorphometric data and will help them in evaluating their biological relevance. 4. Analyzing mouse bone specimen by microCT. Fixed and partially dehydrated mouse long bones and vertebrae will be provided to the Core by project investigators. These specimens will then be scanned using a VivaCT 40 microCT (Scanco Medical, Switzerland).