Morphology and Histology Core

形态学和组织学核心

• 批准号: 8934488
• 负责人: Patricia Florence Ducy
• 金额: $ 30.72万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8934488
• 关键词: Biological; Biomechanics; Brain; Bromodeoxyuridine; Cell Count; Cells; Data; Detection; Endocrine; Equipment; Freezing; Hematoxylin and Eosin Staining Method; Histologic; Histology; Human Resources; Image; Immunohistochemistry; In Situ Nick-End Labeling; Life; Medical; Minerals; Molecular; Morphology; Mus; Osteogenesis; Pancreas; Paraffin; Plastics; Process; Quality Control; Research Personnel; Sampling; Scanning; Skeleton; Specimen; Staining method; Stains; Switzerland; System; Testing; Tissues; Tolonium chloride; Trichrome stain method; base; bone; cresyl violet; long bone; microCT; mouse model; programs; spine bone structure

Project Summary – Core B The three projects of this Program Project rely heavily on the use of mouse models to demonstrate their hypotheses. The purpose of the Morphology and Histology Core is therefore to consolidate equipment and expertise to provide the Investigators of these projects with state-of-the-art, quality controlled histologic and histomorphometric analyses of bone, pancreas and brain specimen as well as microCT and biomechanical analyses of mouse bones. The Core contribution to this Program Project will be at 5 levels: 1. Processing mouse specimens for histology and histomorphometry. Fixed and partially dehydrated mouse skeletons or pancreata will be provided to the Core by project investigators. These samples will then be embedded in plastic medium (vertebrae and long bones) or paraffin (pancreas). Fixed mouse brain specimen will be provided to the Core and either be sectioned without embedding or cryo-embedded and cryo-sectioned. 2. Performing specific stainings and immunostainings of mouse tissues sections. Classic histology stainings such as Hematoxylin/Eosin, Toluidine Blue, von Kossa/Van Gieson, Goldner trichrome, Cresyl violet as well as more specific reactive ones such as TUNEL and TRAP stainings will be performed by the Core personnel at the request of investigators. The Core will also perform immunohistochemistry studies using fluorescent and enzymatic detection. 3. Performing histomorphometric analyses of mouse bone, pancreas and brain specimens. The OsteoMeasure Histomorphometry System (OsteoMetrics Inc., Decatur, GA) will be used to assess static parameters, such as bone volume, trabecular number and cell counts, and dynamic parameters of bone formation, such as mineral apposition rates, from non-decalcified bone sections. It will also be used to quantify -cell mass in pancreas and TUNEL or BrdU positive cells in brain sections. Image J will be used for all other quantifications. The Core will provide investigators with histomorphometric data and will help them in evaluating their biological relevance. 4. Analyzing live mice and mouse bone specimen by microCT. Anesthesized mice or fixed and partially dehydrated mouse long bones and vertebrae will be provided to the Core by project investigators. These specimens will then be scanned using a VivaCT 40 microCT (Scanco Medical, Switzerland). 5. Performing biomechanical tests on mouse bone specimen. Flash frozen mouse long bones and vertebrae will be provided to the Core by project investigators. These samples will then be subjected to compression and 3-point bending tests.

项目摘要-核心B 本计划项目的三个项目在很大程度上依赖于使用小鼠模型来证明其 假设因此，形态学和组织学核心的目的是巩固设备， 专业知识，为这些项目的研究者提供最先进的、质量受控的组织学和 骨、胰腺和脑标本组织形态计量学分析以及microCT和生物力学 老鼠骨头的分析对该计划项目的核心贡献将分为5个级别： 1.处理用于组织学和组织形态测定的小鼠标本。固定和部分脱水小鼠 骨骼或胰腺将由项目研究者提供给核心。这些样本将被 包埋在塑料介质（椎骨和长骨）或石蜡（胰腺）中。固定小鼠脑 将向核心提供标本，切片时不包埋或冷冻包埋， 冷冻切片 2.对小鼠组织切片进行特异性染色和免疫染色。经典组织学染色 例如苏木精/曙红、甲苯胺蓝、vonKossa/货车Gieson、Goldner三色、甲酚紫 以及更特异的反应性染色，如TUNEL和TRAP染色，将由核心细胞进行。 应调查人员的要求。核心还将使用以下方法进行免疫组织化学研究： 荧光和酶检测。 3.对小鼠骨骼、胰腺和脑标本进行组织形态计量学分析。的 OsteoMeasure组织形态测量系统（OsteoMeasure Inc.，Decatur，GA）将用于评估静态 参数，如骨体积、骨小梁数量和细胞计数，以及骨的动态参数 非脱钙骨切片中的矿物质沉积率等。它还将用于 定量胰腺中的β-细胞质量和脑切片中的TUNEL或BrdU阳性细胞。将使用图像J 所有其他量化。Core将为研究者提供组织形态学数据， 评估其生物学意义。 4.用microCT分析活体小鼠和小鼠骨标本。麻醉小鼠或固定和部分 脱水小鼠长骨和椎骨将由项目研究者提供给核心。这些 然后使用VivaCT 40 microCT（Switzerland，Switzerland）扫描标本。 5.对小鼠骨标本进行生物力学测试。速冻小鼠长骨和椎骨 将由项目调查人员提供给核心小组。然后对这些样品进行压缩 和三点弯曲测试。