Morphology and Histology Core

形态学和组织学核心

• 批准号: 8934488
• 负责人: Patricia Florence Ducy
• 金额: $ 30.72万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8934488
• 关键词: Biological; Biomechanics; Brain; Bromodeoxyuridine; Cell Count; Cells; Data; Detection; Endocrine; Equipment; Freezing; Hematoxylin and Eosin Staining Method; Histologic; Histology; Human Resources; Image; Immunohistochemistry; In Situ Nick-End Labeling; Life; Medical; Minerals; Molecular; Morphology; Mus; Osteogenesis; Pancreas; Paraffin; Plastics; Process; Quality Control; Research Personnel; Sampling; Scanning; Skeleton; Specimen; Staining method; Stains; Switzerland; System; Testing; Tissues; Tolonium chloride; Trichrome stain method; base; bone; cresyl violet; long bone; microCT; mouse model; programs; spine bone structure

Project Summary – Core B The three projects of this Program Project rely heavily on the use of mouse models to demonstrate their hypotheses. The purpose of the Morphology and Histology Core is therefore to consolidate equipment and expertise to provide the Investigators of these projects with state-of-the-art, quality controlled histologic and histomorphometric analyses of bone, pancreas and brain specimen as well as microCT and biomechanical analyses of mouse bones. The Core contribution to this Program Project will be at 5 levels: 1. Processing mouse specimens for histology and histomorphometry. Fixed and partially dehydrated mouse skeletons or pancreata will be provided to the Core by project investigators. These samples will then be embedded in plastic medium (vertebrae and long bones) or paraffin (pancreas). Fixed mouse brain specimen will be provided to the Core and either be sectioned without embedding or cryo-embedded and cryo-sectioned. 2. Performing specific stainings and immunostainings of mouse tissues sections. Classic histology stainings such as Hematoxylin/Eosin, Toluidine Blue, von Kossa/Van Gieson, Goldner trichrome, Cresyl violet as well as more specific reactive ones such as TUNEL and TRAP stainings will be performed by the Core personnel at the request of investigators. The Core will also perform immunohistochemistry studies using fluorescent and enzymatic detection. 3. Performing histomorphometric analyses of mouse bone, pancreas and brain specimens. The OsteoMeasure Histomorphometry System (OsteoMetrics Inc., Decatur, GA) will be used to assess static parameters, such as bone volume, trabecular number and cell counts, and dynamic parameters of bone formation, such as mineral apposition rates, from non-decalcified bone sections. It will also be used to quantify -cell mass in pancreas and TUNEL or BrdU positive cells in brain sections. Image J will be used for all other quantifications. The Core will provide investigators with histomorphometric data and will help them in evaluating their biological relevance. 4. Analyzing live mice and mouse bone specimen by microCT. Anesthesized mice or fixed and partially dehydrated mouse long bones and vertebrae will be provided to the Core by project investigators. These specimens will then be scanned using a VivaCT 40 microCT (Scanco Medical, Switzerland). 5. Performing biomechanical tests on mouse bone specimen. Flash frozen mouse long bones and vertebrae will be provided to the Core by project investigators. These samples will then be subjected to compression and 3-point bending tests.

项目总结 – 核心 B 本计划项目的三个项目很大程度上依赖于使用小鼠模型来展示其 假设。因此，形态学和组织学核心的目的是整合设备和 专业知识为这些项目的研究人员提供最先进的、质量控制的组织学和 骨、胰腺和脑标本的组织形态计量学分析以及显微 CT 和生物力学 小鼠骨骼分析。该计划项目的核心贡献将分为 5 个级别： 1. 处理小鼠标本进行组织学和组织形态测定。固定且部分脱水的小鼠 骨骼或胰腺将由项目研究人员提供给核心。这些样本随后将被 嵌入塑料介质（椎骨和长骨）或石蜡（胰腺）中。固定小鼠大脑 样本将被提供给核心并在不包埋或冷冻包埋的情况下进行切片 冷冻切片。 2. 对小鼠组织切片进行特异性染色和免疫染色。经典组织学染色 例如苏木精/曙红、甲苯胺蓝、von Kossa/Van Gieson、Goldner 三色、甲酚紫等 以及更特异的反应性染色，例如 TUNEL 和 TRAP 染色，将由 Core 进行 应调查人员的要求，派出人员。核心还将使用以下方法进行免疫组织化学研究 荧光和酶检测。 3. 对小鼠骨、胰腺和脑标本进行组织形态学分析。这 OsteoMeasure 组织形态测量系统（OsteoMetrics Inc.，Decatur，GA）将用于评估静态 参数，如骨体积、小梁数量和细胞计数以及骨的动态参数 来自非脱钙骨切片的形成，例如矿物质沉积率。它还将用于 量化胰腺中的  细胞质量以及脑切片中的 TUNEL 或 BrdU 阳性细胞。将使用图像 J 对于所有其他量化。核心将为研究人员提供组织形态测量数据，并将帮助 他们评估其生物学相关性。 4. 通过 microCT 分析活体小鼠和小鼠骨骼标本。麻醉或部分固定的小鼠 脱水的小鼠长骨和椎骨将由项目研究人员提供给核心。这些 然后使用 VivaCT 40 microCT（Scanco Medical，瑞士）扫描标本。 5. 对小鼠骨骼标本进行生物力学测试。速冻小鼠长骨和椎骨 将由项目调查员提供给核心。然后这些样本将受到压缩 和三点弯曲测试。