Mays Cancer Center at UT Health SA

项目摘要--流式细胞仪共享资源 FCSR已成为癌症中心支持的共享资源 1991年。FCSR的任务是提供高通量、多维分析和细胞分选 为德克萨斯大学圣安东尼奥分校梅斯癌症中心(MCC)成员提供最先进的设备 (UT Health SA)调查人员和圣安东尼奥地区的其他人。FCSR占地800多平方米。英国《金融时报》的 实验室空间，MCC成员可以方便地进入。FCSR服务包括多参数 细胞亚群的分析和分选；细胞周期分析；下游单细胞克隆和分选 基因组学/转录学.活性氮和氧物种(NOS和ROS)、pH、Ca++的测量 不同活性染料的助熔剂和助熔剂；线粒体损伤的研究；以及活化的测定 半胱氨酸酶。FCSR还提供咨询服务，包括实验设计、仪器培训和 帮助撰写助学金和手稿(包括准备数字、方法章节和预算)。 FCSR为MCC成员提供了两台流式细胞仪细胞分选仪、四台传统分析仪、一台 光谱分析仪，以及最近的质量细胞仪。FCSR由科学总监迈克尔·伯顿领导， 博士，微生物学、免疫学和分子遗传学副教授。Karla Gorena女士是一位 FCSR技术总监兼日常运营经理。Nameer Kirma博士，副教授 分子医学，2018年加入FCSR，担任第二位技术总监，监督太阳神质量 细胞仪。FCSR由德克萨斯大学副总统健康保障办公室提供行政支持 研究(VPR)和MCC。2018年，35个获得同行审查资金的MCC成员使用了FCSR。在……里面 此外，在上一个报告所述期间(2014-2018年)，FCSR为53份与癌症有关的出版物做出了贡献。

Project Summary—Flow Cytometry Shared Resource The Flow Cytometry Shared Resource (FCSR) has been a Cancer Center-supported Shared Resource since 1991. The mission of the FCSR is to deliver high-throughput, multi-dimensional analysis and cell sorting using state-of-the-art equipment for Mays Cancer Center (MCC) members, University of Texas Health San Antonio (UT Health SA) investigators, and others in the San Antonio area. The FCSR occupies over 800 sq. ft. of laboratory space and is conveniently accessible by MCC members. FCSR services include multiparameter analysis and sorting of subpopulations of cells; cell cycle analysis; single cell cloning and sorting for downstream genomics/transcriptomics; measurements of reactive nitrogen and oxygen species (NOS and ROS), pH, Ca++ fluxes and fluxes of different vital dyes; study of mitochondrial damage; and determination of activated caspases. The FCSR also offers consultative services including experimental design, instrument training, and assistance with grant and manuscript writing (including preparation of figures, methods sections, and budgets). The FCSR provides MCC members access to two flow cytometry cell sorters, four conventional analyzers, a spectral analyzer and, most recently, a mass cytometer. The FCSR is led by Scientific Director Michael Berton, Ph.D., Associate Professor of Microbiology, Immunology & Molecular Genetics. Ms. Karla Gorena is a Technical Director and daily operations manager of the FCSR. Nameer Kirma, Ph.D., Associate Professor of Molecular Medicine, joined the FCSR as a second Technical Director in 2018 to supervise the Helios mass cytometer. The FCSR is administratively supported by the UT Health SA Office of the Vice President for Research (VPR) and the MCC. During 2018, 35 MCC members with peer-reviewed funding used the FCSR. In addition, the FCSR contributed to 53 cancer-related publications during the last reporting period (2014-2018).