Mass Spectometry and Proteomics

质谱和蛋白质组学核心概述 质谱学的发展，主要是电喷雾离子化（ESI）液相色谱-质谱 LCMS与串联质谱（MS/MS）的结合，推动了 蛋白质组学和代谢组学领域。因为蛋白质组学和代谢组学测量的是 细胞和动物的状态，它们是研究和发现一系列机制的强大技术。 从癌症到免疫学的病理生理状态。质谱仪的主要目标和 蛋白质组学核心是为VCIIID-COBRE研究人员提供基于质量的实验支持 蛋白质组学和代谢组学领域的光谱分析。我们提供支持 实验设计、样品制备、数据采集、数据分析和所得数据的解释。 服务包括蛋白质组学分析，重点是蛋白质定量和翻译后 修饰、小分子定量、代谢物模式鉴定和速率测量， 使用代谢物和蛋白质的稳定同位素的动力学。这些服务基于40多年来 核心总监马修斯博士在这些领域的领导核心设施的经验。质量 光谱和蛋白质组学核心一直是关键的VCIID-COBRE教师的成功，通过贡献 在VCIID-COBRE计划的第一和第二阶段，为出版物和赠款提供关键信息。与 在第二阶段结束时，增加了高分辨率的沃茨Xevo G2-XS ESI-LCMS/MS四极杆飞行时间仪器和沃茨nanoAcquity UPLC，核心已经能够 扩大其能力和服务进入第三阶段。第三阶段的核心目标是（1）提供 国家的最先进的质谱，尖端技术，和经验丰富的人员，以提高 关于VCIIID-COBRE的研究除了已经进行的标准蛋白质分析外， 教师们已经确定了他们未来研究所需的新技术。这些包括 开发基础广泛的代谢组学测量平台和代谢物 动力学和途径使用稳定的同位素标记示踪剂和扩大我们的能力， 使用稳定同位素标记对难以定义的翻译后修饰进行鉴定和定量 和蛋白质周转率。(2)为VCIIID-COBRE教师提供咨询服务的基础设施 在实验的设计，进行和解释，以促进他们的研究和（3）制定一个长期的- 通过扩大用户基础，建立收费服务结构，加强体制， 支持和适应研究需求的变化。

SUMMARY for the Mass Spectrometry and Proteomics Core Developments in mass spectrometry, primarily electrospray ionization (ESI) liquid chromatography-mass spectrometry (LCMS) with tandem mass spectrometry (MS/MS) has powered the remarkable growth in the fields of both proteomics and metabolomics. Because proteomics and metabolomics measure the dynamic state of cells and animals, they are powerful techniques for studying and discovering mechanisms for a range of pathophysiologic states from cancer to immunology. The primary goal of the Mass Spectrometry and Proteomics Core is to provide VCIID-COBRE investigators with support for experiments based on mass spectrometry-based analyses in the areas of proteomics and metabolomics. We provide support for experimental design, sample preparation, data acquisition, data analysis, and interpretation of data obtained. Services include proteomic analyses with a focus on quantification of proteins and posttranslational modifications, small molecule quantification, metabolite pattern identification, and measurement of rates and kinetics using stable isotopes of both metabolites and proteins. These services are based upon over 40 years of experience in these areas leading core facilities by the Core Director, Dr. Matthews. The Mass Spectrometry and Proteomics Core has been pivotal to the success of VCIID-COBRE faculty by contributing critical information for publications and grants during Phases I and II of the VCIID-COBRE program. With instrumentation expansion at the end of Phase II with the addition of a high resolution Waters Xevo G2-XS ESI-LCMS/MS quadrupole-time of flight instrument and Waters nanoAcquity UPLC, the Core has been able to expand its capabilities and services going into Phase III. The aims of the Core for Phase III are (1) to provide state-of-the-art mass spectrometry, cutting-edge techniques, and experienced personnel to enhance the research of the VCIID-COBRE. In addition to the standard protein analyses already conducted, VCIID-COBRE faculty has identified new techniques that are required for their future investigations. These include development of a broad-based platform for metabolomics measurements and measurement of metabolite kinetics and pathways using stable isotopically labeled tracers and broadening our capabilities to provide identification and quantification using stable isotope labels of difficult to define posttranslational modifications and rates of protein turnover. (2) To provide an infrastructure of consultative services to VCIID-COBRE faculty in the design, conduct, and interpretation of experiments to facilitate their research and (3) To develop a long- term plan for sustainability through expansion of user base with a fee-for-service structure, strong institutional support, and adaptation to changes in research needs.