Neural basis of memory in primate medial temporal lobe

项目摘要 内侧颞叶(MTL)在快速形成情景记忆中起关键作用。 人类和非人类灵长类动物，而在自由活动的啮齿动物身上进行的研究也有类似的 确定了这些相同的结构对空间导航至关重要。这些工作中的每一项 反映强大的研究传统，这些传统对我们对医学的理解做出了重大贡献 时间叶功能，但问题仍然是如何协调他们的大量数据集。一 令人信服的假设是，支持MTL在空间中的作用的神经机制 导航也支持情节记忆的形成。我们提出了一套创新的 为直接验证这一假说而设计的实验我们的方法包括记录神经活动 在两种情况下，来自绒猴内侧颞叶的相同神经元。一 让受试者戴着头巾，执行人类特有的识别记忆任务 而在其他自由移动的受试者中，实验则在常用的空间环境中导航 在啮齿动物的研究中。我们的创新方法将使我们能够首次测试 灵长类内侧颞叶相同神经元在典型任务中的支持行为 这两种研究传统。目标1试图用以下方法来表征绒猴的识别记忆 就像空间导航一样，这项任务依赖于附带的记忆形成。Aim 2的实验 研究海马CA区和内嗅皮层在空间导航中的作用，包括 位置单元和格子单元的假定存在。目标3直接测试主体 对这一提议的质疑，通过在受试者执行 目标1中的识别记忆任务，以及类似于目标2中的研究的空间环境导航。

Project Summary The medial temporal lobe (MTL) plays a critical role in the rapid formation of episodic memories in human and nonhuman primates, while research performed in freely-moving rodents has likewise identified these same structure as being pivotal for spatial navigation. Each of these lines of work reflect powerful research traditions that have significantly contributed to our understanding of medial temporal lobe function, but questions remain about how to reconcile their considerable data sets. One compelling hypothesis is that the same neural mechanisms that support the role of MTL in spatial navigation also support the formation of episodic memories. We propose an innovative set of experiments designed to directly test this hypothesis Our approach involves recording neural activity from the same neurons in the medial temporal lobe of marmoset monkeys in two contexts. One involves head-restrained subjects performing a recognition memory task typical of human experiments while in the other freely-moving subjects navigate spatial environments commonly used in studies of rodents. Our innovative approach will allow us to test – for the first time - whether the same neurons in primate medial temporal lobe support behaviors performed in tasks representative of these two research traditions. Aim 1 seeks to characterize recognition memory in marmosets using a task that – like spatial navigation – relies on incidental memory formation. Experiments in Aim 2 examine the role of the hippocampal CA fields and entorhinal cortex in spatial navigation, including the putative existence of place cells and grid cells, respectively. Aim 3 directly tests the principal question of this proposal by recording from the identical neurons while subjects perform the recognition memory task in Aim 1 and navigate spatial environments similar to studies in Aim 2.