Mouse Core

这个核心实验室的总体目标是提供基础设施、材料、动物、技术专长 以及将促进在检查艾滋病毒的研究中使用人源化免疫缺陷小鼠的支持 活体内的蓄水池。最近的进展使缺乏自身免疫系统的小鼠能够 植入人体组织。能够发育成人类免疫细胞的人体组织被放置在 小鼠在那里发育成多种类型的人类免疫细胞。使用的人体组织 在这些研究中能够在老鼠和人类身上进行高度的操纵和实验 与小鼠细胞相比，细胞更容易感染艾滋病毒。这提供了一个强大的模型来 检查人类血细胞发育，研究艾滋病毒感染对人类细胞的影响，并检查 从免疫细胞中清除艾滋病毒的方法。这些人源化的小鼠的一代是高度专业化的 程序中，由于要求免疫缺陷的小鼠品系、人的造血组织具有感染性 在该系统中进行实验所需的材料、专门设备以及必要的技能和知识。 作为核心项目的负责人，这一新的核心项目将是该项目的一项独特而宝贵的资源 拥有超过24年的人性化鼠标模型经验和核心员工的帮助 在这些模型中开创各种新技术和新发现。为了让我们能够准确地评估和 比较我们的结果，老鼠模型的标准化变得绝对关键。因此，它是必不可少的 这个程序，为了得出正确的结论，涉及人源化小鼠的研究 实验室之间的标准化。这个核心的中心目的是提供专门的服务和 本计划中的所有3个项目所需的资源，以标准化方式生成 一致可靠的数据。

The overall goal of this Core laboratory is to provide the infrastructure, materials, animals, technical expertise and support that will facilitate the use of humanized immunodeficient mice in studies that examine HIV reservoirs in vivo. Recent advances have enabled the use of mice that lack their own immune system mice to be implanted with human tissue. Human tissue capable of developing into human immune cells is placed in the mice where it undergoes development into multiple types of human immune cells. The human tissue used in these studies is capable of a high degree of manipulation and experimentation in the mouse and the human cells, versus the mouse cells, are further susceptible to infection with HIV. This provides a powerful model to examine human blood cell development, to study the effects of HIV infection on human cells, and examine ways to purge HIV from immune cells. The generation of these humanized mice is a highly specialized procedure, due to the requirement for immunodeficient mouse strains, human hematopoietic tissue, infectious material, specialized facilities, and the necessary skill and knowledge to perform experiments in this system. This new core would represent a unique and valuable resource for this program, as the director of the Core has greater than 24 years of experience with humanized mouse models and the staff of the core have helped pioneer various novel techniques and findings in these models. In order for us to accurately assess and compare our results, standardization of the mouse model becomes absolutely critical. Thus, it is essential for this program, in order to draw the proper conclusions, that the studies involving humanized mice be standardized between laboratories. The central purpose of this core is to provide the specialized services and resources needed by all 3 projects in this program in the standardized fashion necessary to generate consistent and reliable data.