Flow Cytometry Core

项目总结 莫菲特癌症中心(MCC)的流式细胞仪核心设备(FCC)提供集中和切割- 为MCC成员的癌症相关研究提供边缘流式细胞仪服务。流式细胞术是一种 为MCC的细胞生物学、免疫学和翻译研究工作提供了不可或缺的分析工具。目标 FCC的目的是为成员提供高技能的工作人员和先进的流式细胞仪技术，这些 提供：1a)在单细胞水平上准确测量和分析多个参数；1b) 能够快速纯化目标人群，评估特定的细胞功能，并确定分子特征 纯化的细胞群体；2)协助实验设计、数据分析和解释以及 准备赠款和手稿；以及3)提供流式细胞术方面的培训和教育 技术和方法。 FCC的科学主任是Julie Djeu博士。联邦通信委员会配备了训练有素的仪器。 在研究型流式细胞仪领域拥有40年以上经验的专家。这个 设施配备了七台台式分析仪，两台细胞分选机，一台多路珠阵列分析仪和一台 自动珠子分拣机。FCC增加了新的仪器设备并升级了系统以扩大其 为会员提供服务，并提供现代流式细胞仪技术。联邦通信委员会使用了这一用途 实验室信息管理系统(LIMS)，以整合使用跟踪、调度和计费 功能。LIMS还为项目和数据管理提供了一个安全的存储库，可供访问 由成员和他们的实验室工作人员。在此期间，FCC为171份同行评议的出版物做出了贡献 审查期与前一审查期的112个相比。在最近一个财年，FCC提供了 为53个成员提供服务，同行评议资助的成员占总使用量的89%。

PROJECT SUMMARY The Flow Cytometry Core Facility (FCC) of the Moffitt Cancer Center (MCC) provides centralized and cutting- edge flow cytometry services for MCC members for their cancer-related research. Flow cytometry is an indispensable analytical tool for cell biology, immunology, and translational research efforts at MCC. The goals of the FCC are to support members with highly skilled staff and advanced flow cytometry technologies, which provide: 1a) Accurate measurements and analyses of multiple parameters at the single-cell level; 1b) The ability to rapidly purify target populations, to assess specific cellular functions, and to molecularly characterize purified cell populations; 2) Assistance in experimental design, data analysis and interpretation, as well as with the preparation of grants and manuscripts; and 3) Delivery of training and education in flow cytometry technologies and methodologies. The Scientific Director of the FCC is Julie Djeu, PhD. The FCC is staffed with highly trained instrument specialists who have more than 40 years in combined experience in research-based flow cytometry. The facility is equipped with seven benchtop analyzers, two cell sorters, a multiplex bead array analyzer, and an automated bead sorter. The FCC has added new instrumentation and upgraded systems to expand its services to members and to provide access to modern flow cytometry technologies. The FCC employs the use of a Laboratory Information Management System (LIMS) to consolidate usage tracking, scheduling, and billing functions. The LIMS also provides a secure repository for project and data management, which is accessible by members and their laboratory staff. The FCC has contributed to 171 peer-reviewed publications during this review period compared with 112 the previous review period. In the most recent fiscal year, the FCC provided service for 53 members, with 89% of total usage for peer-review-funded members.