Animal Imaging

摘要 Animal Imaging Core为个体项目提供非侵入性、高分辨率的定量成像- 基于代谢，表型和遗传表征肿瘤及其 微环境，包括用于人类癌症啮齿动物模型的肿瘤细胞的体内运输。这是 通过监测“直接靶向”探针和单模态和多模态的表达来完成。 使用光学（生物发光和荧光）、放射性核素（PET、SPECT和 放射自显影）、MRI/MRS、CT和US成像。这些非侵入性成像技术长期以来一直是 在MSK建立的小动物成像研究，是定量的，因此很容易适应纵向 问题研究ZRC动物饲养场内所有动物成像能力的共同定位提供了增强的 生物安全和功能，并容纳核心的焦点120 microPET和Imveon microPET/microCT，Ivis Spectrum和Spectrum CT光学成像系统NanoSPECT/CT Plu和Vevo 2100超声系统。 Ivis 100光学成像系统已升级为Lumina II系统，并保留在12楼。 洛克菲勒研究实验室，为该大楼的研究人员提供方便。一个新 等中心，图像（CT）引导微辐照器，X-RAD 225 Cx图像引导生物辐照器，用于精确 (down至1-mm 3）的啮齿动物肿瘤和其他结构的治疗照射为研究人员提供了 在空间准确度和剂量输送精确度方面具有临床级放射治疗能力。该芯体的 7.0T 30-cm钻孔Bruker NMR成像和光谱系统位于ZRC Vivarium内。新的PET 插入（由RC 2CA 14897资助），核心最近收到的是与7 T磁铁兼容， 目前使用。此外，核心有一个11.7T的垂直磁铁，并将很快接受一个新的9.4T磁铁 （由1 S10 OD 023669资助），配备更新的控制台（AV 3），以取代旧的4.7T MR系统） 在这个空间也是如此。核心还为调查人员提供关键辅助设备， 服务，如富士胶片BAS-1800 II荧光板数字放射自显影系统，数码相机， 配备Olympus B 201荧光显微镜（带电动载物台）和Microm HM 500 M低温恒温器 切片机和软件，如用于线性和非线性房室建模的SAAM II，用于体素的PMOD， 基于房室模型/参数成像和OLINDA的内部放射性核素辐射剂量测定。 这一最先进的动物成像核心的科学价值和成本效益由 以下（除其他指标外）：2013-2017年近500篇同行评审的核心支持出版物和9篇 主要仪器由NIH和其他共享仪器赠款提供的资金支持购买。

ABSTRACT The Animal Imaging Core provides individual projects with non-invasive, high-resolution quantitative imaging- based capabilities for metabolic, phenotypic, and genetic characterization of tumors and their microenvironment, including in vivo trafficking of tumor cells for rodent models of human cancer. This is accomplished by monitoring of “directly-targeting” probes and of the expression of single-and multi-modality reporter genes using optical (bioluminescence and fluorescence), radionuclide (PET, SPECT, and autoradiography), MRI/MRS, CT, and US imaging. These non-invasive imaging techniques have long been established at MSK for small-animal imaging studies, are quantitative and thus readily adaptable to longitudinal studies. Co-localization of all animal imaging capabilites within the ZRC vivarium provides enhanced biosecurity and functionality and houses the Core’s Focus 120 microPET and Imveon microPET/microCT, Ivis Spectrum and Spectrum CT optical imaging systems NanoSPECT/CT Plu, and Vevo 2100 ultrasound system. The Ivis 100 optical imaging system has been upgraded to a Lumina II system and remains on the 12th floor of the Rockefeller Research Laboratory to provide ready access for investigators in that building. A new isocentric, image (CT)-guided microirradiator, the X-RAD 225Cx Image-Guided Biological Irradiator, for precise (down to 1-mm3) therapeutic irradiation of rodent tumors and other structures provides investigators with clinical-grade radiotherapy capability in terms of spatial accuracy and precision of dose delivery. The Core’s 7.0T 30-cm boreBruker NMR imaging and spectroscopy systems is within the ZRC Vivarium. The new PET insert (funded by RC2CA14897), which the Core recently received is compatible with the 7T magnet and is in current use. Additionally, the Core has an 11.7T vertical magnet and will shortly accept a new 9.4T magnet (funded by 1S10 OD023669) with an updated console (AV3) to replace the old 4.7T MR system) to be installed in this space as well. The Core also provides investigators with access to critical ancillary equipment and services such as a Fuji Film BAS-1800II phosphor-plate digital autoradiography system, a digital-camera- equipped Olympus B 201 fluorescence microscope with motorized stage, and a Microm HM500M cryostatic microtome and software such as SAAM II for linear and non-linear compartmental modeling, PMOD for voxel- based compartmental modeling/ parametric imaging, and OLINDA for internal radionuclide radiation dosimetry. The scientific value and cost-effectiveness of this state-of-the-art Animal Imaging Core is documented by the following (among other metrics): nearly 500 peer-reviewed Core-supported publications from 2013-2017 and 9 major instruments purchased with funding support provided by NIH and other Shared Instrumentation grants.