High-Throughput Screening and Computational Chemistry Core

项目摘要/摘要：高通量筛选和计算化学核心(核心1) 高通量筛选和计算化学核心(HTSCC)的目标是 为内布拉斯加州分子靶标发现和开发中心(MTDD)的使命提供 所需的关键功能基础设施、试剂、仪器、技术专长和培训 MTDD调查人员开展他们的研究项目。通过进行高通量siRNA、化学和 在计算机筛选实验中，研究人员有了一种新颖而强大的工具来快速高效地进行 产生关于基因途径和功能的准确信息，以及识别小分子 抑制剂可以作为可逆的化学探针来剖析这些信号通路，以及 识别用于药物发现的先导化合物的可能性。这些活动通常不适用于 因费用或缺乏专业技术技能和/或缺乏专门技术而开展的单个研究实验室 设备。对于对使用HTS和高内容成像(HCI)服务感兴趣的MTDD调查人员， 能够增加自动化、小型化分析和整合专为高通量设计的仪器 可以大大降低大量筛选和非筛选的试剂、塑料器皿和人力成本 在他们的实验室里进行的实验。另外一个好处是，调查员的范围 可以极大地扩大实验范围并以更高的精度执行，通常会增强 数据的质量和再现性。在整个过程中，现场工作人员提供专业知识、培训、支持、 以及筛选技术性能方面的反馈，从而最大限度地降低成本、提高吞吐量，以及 最大限度地生成可解释的结果。此外，核心还将不断开发和实施 根据需要采用新技术，并努力获得和/或升级必要的仪器设备。通过这些手段， 核心将有助于使MTDD调查人员在研究资金方面更具竞争力，并促进长期的 进行最终改善患者健康的研究的长期目标。

Project Summary/Abstract: High-Throughput Screening and Computational Chemistry Core (Core 1) The objective of the High-Throughput Screening and Computational Chemistry Core (HTSCC) is to contribute to the mission of the Nebraska Center for Molecular Target Discovery and Development (MTDD) by providing the critical functional infrastructure, reagents, instrumentation, technical expertise, and training necessary for MTDD investigators to conduct their research projects. By conducting high-throughput siRNA, chemical, and virtual in silico screening experiments, investigators have a novel and powerful tool to rapidly and efficiently generate accurate information about gene pathways and functions, as well as to identify small molecule inhibitors that can serve as reversible chemical probes to dissect these signaling pathways, along with the possibility of identifying lead compounds for drug discovery. These activities are generally not practical for the individual research laboratory to carry out for reasons of cost or lack of specialized technical skills and/or equipment. For MTDD investigators interested in using HTS and high-content imaging (HCI) services, the ability to add automation, miniaturize assays, and incorporate instrumentation designed for high-throughput can greatly reduce reagent, plastic-ware, and labor costs for a vast array of screening and non-screening experimentation performed in their laboratories. An additional benefit is that the scope of investigator experimentation can be greatly broadened and performed with greater precision, typically enhancing the quality and reproducibility of data. Throughout this process, on-site staff provides expertise, training, support, and feedback in the performance of screening technologies that will minimize costs, enhance throughput, and maximize the generation of interpretable results. Moreover, the core will continually develop and implement new technologies as needed and work to obtain and/or upgrade necessary instrumentation. By these means, the core will help to make MTDD investigators more competitive for research funding and to facilitate the long- term goal of performing research that ultimately results in the improvement of the health of patients.