Proteomics and Metabolomics Shared Resource

蛋白质组学与代谢组学资源共享 项目摘要 生物分析实验室共享资源在前一次审查中被评为“优秀”。以反映 维克森林浸信会综合癌症中心(WFBCCC)成员不断变化的需求，自那以来 重组形成蛋白质组和代谢组学共享资源(PMSR)。此共享资源 为全球和地区提供先进的质谱学服务、技术和科学咨询 靶向蛋白质组学、代谢组学和脂类组学研究。PMSR专门从事发现、识别、 生物分子的表征和定量，包括蛋白质、脂类和其他代谢物的质量 各种生物样品中的光谱和生化方法；蛋白质的层析， 肽、脂、核酸和化合物；生物能量新陈代谢的测量；和有针对性的 使用免疫分析的多重分析。PMSR的资产包括1)三个主要的质谱学系统 用于代谢组学和脂类组学分析，包括新的岛津LC MS-8050 LC/MS/MS系统；2)A 新安装的数据独立采集和并行的Thermo Q-Exactive HF质谱仪 与用于蛋白质组学分析的NanoLC系统连接的反应监测能力；3)访问广泛的 RTI代谢组学的一系列仪器和专业知识，现在是与WFBCCC广泛合作的一部分 和PMSR；4)人员的专业知识。PMSR由克里斯蒂娜·M·福尔杜伊博士和马克·O执导。 Liavy博士，目前有四名员工(李静云博士，吴汉志博士，Brian Fulp，B.S.， 和马克·莫里斯，B.S.)总计2.7 FTE(请求1.15个CCSG FTE)。在上一次完整的 在报告年度(2014年11月1日-2015年10月31日)，PMSR支持了维克大学24名教职员工的研究 森林浸信会医疗中心和6名外部研究人员，分析了2,116个样本(约90% PMSR容量)。在此期间，获得同行审查资金的WFBCCC成员的使用率为47%(75% 基于样本数量)。该机构对PMSR的支持体现在其投资接近 100万美元用于购买新仪器和雇用额外人员，这是一笔相当大的成本分摊， 增加CCSG在PMSR的投资价值。共享资源每年由 机构的中心和核心咨询委员会，该委员会隶属于生物医学研究服务和 行政管理，并向院长报告。此外，共享资源董事的季度会议包括 举行并包括有关活动、最佳做法、增强功能和管理的报告/讨论 管理技巧。这些会议的主席是渡边幸之介博士，他是Shared的副主任 资源。WFBCCC高级领导层有最终责任评估 PMSR支持癌症中心的使命。

PROTEOMICS AND METABOLOMICS SHARED RESOURCE Project Summary The Bioanalytical Laboratory Shared Resource scored as “excellent” during the previous review. To reflect the evolving needs of Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) members, it has since been restructured to form the Proteomics and Metabolomics Shared Resource (PMSR). This Shared Resource provides advanced mass spectrometry services, technologies, and scientific consultation for global and targeted proteomics, metabolomics and lipidomics studies. The PMSR specializes in discovery, identification, characterization, and quantification of biomolecules, including proteins, lipids and other metabolites by mass spectrometry and biochemical methods in a variety of biological specimens; chromatography of proteins, peptides, lipids, nucleic acids and chemical compounds; measurement of bioenergy metabolism; and targeted multiplex analysis using immunoassays. Assets of PMSR include 1) Three major mass spectrometry systems for metabolomics and lipidomics analysis, including a new Shimadzu LCMS-8050 LC/MS/MS System; 2) A newly-installed Thermo Q-Exactive HF mass spectrometer with data-independent acquisition and parallel reaction monitoring capabilities interfaced with a nanoLC system for proteomics analysis; 3) Access to a broad range of instruments and expertise at RTI Metabolomics, now part of a broad partnership with the WFBCCC and PMSR; and 4) Expertise of personnel. The PMSR is directed by Cristina M. Furdui, Ph.D., and Mark O. Lively, Ph.D., and currently employs four individuals (Jingyun Lee, Ph.D, Hanzhi Wu, Ph.D., Brian Fulp, B.S., and Mark Morris, B.S.) for a combined total of 2.7 FTE (1.15 CCSG FTEs requested). During the last full reporting year (11/1/2014 – 10/31/2015), the PMSR supported the research of 24 faculty members of Wake Forest Baptist Medical Center and 6 external researchers, analyzing 2,116 samples (approximately 90% of PMSR capacity). Usage by WFBCCC members with peer-reviewed funding during this period was 47% (75% based on number of samples). The Institution's support of the PMSR is reflected in its investment of close to $1M for purchasing of new instrumentation and hiring of additional personnel, a significant cost-sharing that increases the value of the CCSG investment in PMSR. The Shared Resource is reviewed annually by the Institution's Centers and Cores Advisory Committee, which is within Biomedical Research Services and Administration, and reports to the Dean. In addition, quarterly meetings of the Shared Resource Directors are held and include reports/discussions on activity, best practices, enhanced capabilities, and administrative management techniques. These are chaired by Kounosuke Watabe, Ph.D., the Associate Director for Shared Resources. The WFBCCC Senior Leadership has the final responsibility for evaluating the effectiveness of PMSR in supporting the Cancer Center mission.