Replacement Equipment Components for an 800 MHz NMR Spectrometer

描述（由申请人提供）：杜克大学建议用新的控制台和泵送磁铁监测数据记录器替换，更新和增强其800 MHz核磁共振光谱系统。800兆赫光谱仪安置在杜克核磁共振光谱中心并由其管理，该中心为杜克大学的所有大学研究项目以及研究三角和东南地区的大学和政府实验室的科学家提供核磁共振资源。它支持基础生物化学和生物医学的前沿研究。所要求的新设备部件是为了替换10多年前安装在该系统上的原始设备，这些设备现在正在以不可接受的速度故障。这极大地影响了许多研究人员的研究活动，他们依靠杜克800仪器来完成他们的资助项目，并且在安装后的4年内，他们已经在24/7计划的90-95%的时间内使用它。然而，目前，由于关键部件老化导致的故障导致的意外维护和维修，可用时间更加有限。瓦里安Inova控制台和磁体监测系统已不再生产，取而代之的是新一代光谱仪和冷冻监测系统，这使得仪器用于研究的时间受到限制。在某些情况下，我们的系统的新部件不再可用，并且翻新，使用过的部件通常也不能立即可用。我们的800 MHz核磁共振系统所需的新设备组件提供了一种具有成本效益的策略，可以以新系统成本的一小部分将该地区大量使用的独特光谱仪恢复到其设计能力。此外，更换磁体监测系统保护了我们的泵送18.9特斯拉核磁共振磁体价值超过100万美元的投资。目前在以下领域的研究活动将通过杜克800的升级得到加强：蛋白质组装的研究；蛋白质构象动力学与折叠；膜相关脂质及其合成过程中的酶；酶的结构表征与再设计；蛋白质的细胞内核磁共振光谱；与HIV疫苗设计相关的免疫原性肽和磷脂的抗体识别；细胞信号;多维核磁共振数据快速获取方法开发。

DESCRIPTION (provided by applicant): Duke University proposes to replace, update, and enhance ts 800 MHz NMR spectroscopy system with a new console and pumped magnet monitoring data logger. The 800 MHz spectrometer is housed in and managed by the Duke NMR Spectroscopy Center which serves as an NMR resource for all university research projects at Duke and for scientists at universities and government laboratories in the Research Triangle and the Southeastern region. It supports frontier research in basic biochemical and biomedical sciences. The requested new equipment components are needed to replace original equipment installed with the system over 10 years ago, that is now failing at an unacceptable rate. This has significantly impacted research activities of numerous investigators who depend on the Duke 800 instrument to pursue their funded projects and who were already using it in the range of 90-95% of a 24/7 schedule, year around within 4 years of installation. Currently, however, the available time has been more limited due to unanticipated maintenance and repairs associated with failure of critical components as they have aged. This limitation in the instrument time available for research is compounded by the fact that the Varian Inova console and magnet monitoring system are no longer manufactured, having been replaced by a new generation spectrometer and cryogen monitoring system. New parts for our system in some cases are no longer available, and refurbished, used parts are often not immediately available, either. The new equipment components requested for our 800 MHz NMR system provide a cost effective strategy to restore this heavily used and unique spectrometer in this region to its designed capability at a fraction of the cost of a new system. In addition, replacement of the magnet monitoring system protects the investment in our pumped 18.9 Tesla NMR magnet valued at greater than $1M. Current research activities in the following areas will be enhanced by this upgrade to the Duke 800: studies of protein assemblies; protein conformational dynamics and folding; membrane associated lipids and enzymes involved in their synthesis; enzyme structural characterization and redesign; in-cell NMR spectroscopy of proteins; antibody recognition of immunogenic peptides and phospholipids associated with HIV vaccine design; cellular signaling; and methods development for rapid acquisition of multidimensional NMR data.