AB SCIEX QTRAP 5500 LC MS/MS system

描述（由申请人提供）：该NCRR共享仪器提案寻求资金，用于购买Buck年龄研究所的集成HPLC MS/MS系统，该系统由Applied Biosystems/Sciex QTRAP 5500三重四极线性离子阱质谱仪组成，配备纳米电喷雾电离源质谱仪和Eksigent/Tempo纳米毛细管高效液相色谱仪（HPLC）。该仪器将被安置并维护在巴克研究所的化学和蛋白质组学核心，并将服务于至少14个不同的研究项目，其中3个涉及外部机构的研究人员，即UCSF，格拉德斯通研究所和爱荷华大学。简而言之，我们建议使用该仪器专门用于从与人类健康相关的各种生物材料中获得的各种感兴趣的蛋白质衍生的肽的靶向定量，包括小鼠和人体组织，细胞系，人类血浆样本和感染生物体。总的来说，这些分析将主要集中在QTRAP 5500通过多反应监测（MRM）进行多肽多路定量的能力上。此外，我们将使用这种新仪器来识别和量化一组预先选择的生物学上重要的酶和非酶翻译后修饰，使用中性损失扫描和正/负模式切换功能。从所要求的仪器中获得的数据对于理解各种疾病模型和范例至关重要。事实上，这些研究项目包括对神经退行性疾病过程的研究，如亨廷顿氏病、帕金森病和阿尔茨海默病，鉴定用于早期检测癌症的新蛋白靶点或生物标志物，研究线粒体功能障碍在衰老和年龄相关疾病中的作用，以及分析生物膜在人类细菌发病机制中的作用。

DESCRIPTION (provided by applicant): This NCRR shared instrumentation proposal seeks funding for the purchase of an integrated HPLC MS/MS system at the Buck Institute for Age Research consisting of an Applied Biosystems/Sciex QTRAP 5500 triple quadrupole linear ion-trap mass spectrometer equipped with a nano-electrospray ionization source mass spectrometer and an Eksigent/Tempo nano-capillary high performance liquid chromatograph (HPLC). This instrument will be housed and maintained at the Buck Institute's Chemistry & Proteomics Core and will serve a minimum 14 different research projects, 3 of which involve researchers at outside institution, i.e., UCSF, Gladstone Institute and the University of Iowa. In brief, we propose to operate this instrument exclusively for the targeted quantitation of peptides derived form various proteins of interest that are obtained from various biological materials relevant to human health, including mouse and human tissues, cell lines, human plasma sample, and infection organisms. Overall, these analyses will focus primarily on the requested QTRAP 5500's ability to perform multiplexed quantitation of peptides via multiple reaction monitoring (MRM). In addition, we will use this new instrument to identify and quantify a pre-select set of biologically important enzymatic and non-enzymatic posttranslational modifications using neutral loss scanning and positive/negative mode switching capabilities. The data obtained from the requested instrument will be critical to understand various disease models and paradigms. Indeed, these research projects encompass the study of neurodegenerative diseases processes such as Huntington's, Parkinson's and Alzheimer's disease, the identification of novel protein targets or biomarkers for the early detection of cancer, examining the role of mitochondrial dysfunction in aging and age-related diseases, and analysis of the role of biofilms in human bacterial pathogenesis. PUBLIC HEALTH RELEVANCE: New instrumentation is requested that will serve a variety of research projects at the Buck Institute for Age Research and neighboring institutions. The requested mass spectrometer system will allow us to carry out a variety of research projects that encompass the study of neurodegenerative (Huntington's, Parkinson's and Alzheimer's disease), the identification of novel biomarkers for the early detection of cancer, determining the role of mitochondrial dysfunction in aging and age-related diseases, and the analysis of the bacterial biofilms that form in some human diseases, such as chronic ear infections and pelvic inflammatory disease.