950 MHz NMR Spectrometer with Cryogenic Probe

描述(由申请人提供)：由于越来越多的基于生物分子的核磁共振研究项目，NIH资助的马里兰大学巴尔的摩分校(UMB)、马里兰大学巴尔的摩县分校(UMBC)和马里兰大学学院公园分校(UMCP)的首席研究人员对最先进的屏蔽950 MHz核磁共振有科学需求。该仪器将位于UMB核磁共振共享核心设施中专门设计的空间中，该设施已完全准备好容纳该仪器(http://medschool.umaryland.edu/nmr/).马里兰大学已承诺提供必要的资源，以正确安装和运营这一新的共享资源，并为设施经理克里斯汀·瓦尼博士提供工资支持。950 MHz核磁共振的管理和共享操作由一个委员会制定，该委员会包括大卫·J·韦伯(UMB)、迈克尔·萨默斯(UMBC)、大卫·富什曼(UMCP)和克里斯汀·瓦尼(UMB)博士。NIH资助的需要950 MHz核磁共振波谱仪的主要研究人员包括：1.大卫·J·韦伯(UMB)博士，涉及S100蛋白质的蛋白质-蛋白质复合体的结构/功能和抑制；2.迈克尔·萨默斯(UMBC)博士对逆转录病毒基因组包装的核磁共振研究；3.大卫·富什曼(UMCP)博士的溶液结构和多泛素链的动力学；4.夸库·达耶(UMCP)博士(UMCP)分子识别：基因调控和催化中RNA-大分子相互作用的结构和动力学基础；5.布鲁斯·约翰逊(UMBC)博士继续开发和维护NMRview；6.Alex Drohat(UMB)博士，CpG特异性DNA糖基酶的结构；7.Frank MarGolis博士，OMP的结构/功能研究及其对Na？交换器(NCX)的影响；8.James Nataro(UMB)博士，分散素和AAF家族的结构/功能研究；以及9.Kristen Varney(UMB，经理)博士，涉及转移蛋白的蛋白质-蛋白质复合体1.Vitali Tugarinov博士(项目10；标题：“开发核磁共振和同位素标记方法研究高分子量蛋白质的结构和动力学”最近受聘于马里兰大学(UMCP)，因此他和另外12名研究人员(项目11-21)以及新的/有代表性的项目被包括在申请中。还列出了其他14名研究人员的项目(项目22-35)，他们过去曾使用过核磁共振中心，未来可能需要最先进的950 MHz核磁共振光谱仪。与过去一样，我们将应要求向大西洋中部地区以及整个美国的其他私人投资机构提供950兆赫核磁共振。由于NIGMS资助的IMSD博士项目(PI：Michael Summers)，UMBC/UMB生物化学项目中代表不足的少数族裔(URM)的参与率在过去15年中从0%跃升至30%。因此，除了支持本提案中列出的NIH资助的研究项目外，申请的资源还将为马里兰大学的URM尖子生提供最先进的培训设施。 公共卫生相关性：NIH资助的马里兰大学巴尔的摩分校(UMB)、马里兰大学巴尔的摩县分校(UMBC)和马里兰大学学院公园分校(UMCP)正在进行的核磁共振相关研究项目解决了许多重要的公共卫生问题，包括癌症、艾滋病毒/艾滋病、衰老、心脏病、神经科学以及药物设计的几个方面

DESCRIPTION (provided by applicant): NIH-funded principal investigators at the University of Maryland, Baltimore (UMB), the University of Maryland, Baltimore County (UMBC), and the University of Maryland, College Park (UMCP) have scientific need for a state-of-the-art shielded 950 MHz NMR because of an increasing number of biomolecular NMR-based research projects. This instrument will be located in specially designed space in the UMB NMR shared core facility, which is fully ready to house this instrumentation (http://medschool.umaryland.edu/nmr/). The University of Maryland has committed to providing the resources necessary to properly install and operate this new shared resource and salary support for the facility manager, Dr. Kristen Varney. The administration of the 950 MHz NMR and its shared operation are set by a committee that includes Drs. David J. Weber (UMB), Michael Summers (UMBC), David Fushman (UMCP) and Kristen Varney (UMB). The NIH-funded principle investigators (PIs) that have a need for a 950 MHz NMR spectrometer include: 1. Dr. David J. Weber (UMB), Structure/function and inhibition of protein-protein complexes involving S100 proteins; 2. Dr. Michael Summers (UMBC) NMR studies of retroviral genome packaging; 3. Dr. David Fushman (UMCP) Solution structure and dynamics of polyubiquitin chains; 4. Dr. Kwaku Dayie (UMCP) Molecular recognition: structural and dynamic basis for RNA-macromolecular interactions in gene regulation and catalysis; 5. Dr. Bruce Johnson (UMBC) Continued development and maintenance of NMRview; 6. Dr. Alex Drohat (UMB), Structure of CpG specific DNA glycosylases; 7. Dr. Frank Margolis (UMB), Structure/function studies of OMP and its influence on the Na?exchanger (NCX); 8. Dr. James Nataro (UMB), Structure/function studies of dispersin and of the AAF family of fimbraie; and 9. Dr. Kristen Varney (UMB, Manager), Protein-protein complexes involving the metastasis protein 1. Dr. Vitali Tugarinov (project 10; Title: "Development of NMR and isotope labeling methodology for the studies of structure and dynamics of high molecular weight proteins) was recently hired at the University of Maryland (UMCP), so he as well as twelve additional investigators (projects 11-21) with new/representative projects are included in the application. Projects for fourteen other investigators (projects 22-35) who have used the NMR center in the past and could have future need for a state-of-the-art 950 MHz NMR spectrometer are also listed. As in the past, we will make the 950 MHz NMR available to other PIs in the Mid-Atlantic region as well as throughout the United States, upon request. Because of an NIGMS funded IMSD PhD program (PI: Michael Summers), underrepresented minority (URM) participation in the UMBC/UMB Biochemistry program has jumped from 0 to 30% over the past 15 years. Thus, in addition to supporting the NIH funded research projects listed in this proposal and throughout the country, the requested resources will also provide top URM students at the University of Maryland with state-of the art training facilities. PUBLIC HEALTH RELEVANCE: The ongoing NMR-related projects under study by NIH funded principal investigators at the University of Maryland, Baltimore (UMB), University of Maryland, Baltimore County (UMBC), and University of Maryland, College Park (UMCP) address many important public health concerns including cancer, HIV/AIDS, aging, heart disease, neuroscience, and several aspects of drug design