In vivo multiphoton microscope for UT Southwestern Live Cell Imaging Facility

描述（由申请人提供）：我们正在为UT西南活细胞成像设施（LCIF）申请体内多光子显微镜，该设施是一个校园范围内的核心设施，为来自达拉斯UT西南医学中心校园35个部门的150多名研究人员提供光学显微镜仪器。该仪器将提供访问技术，目前是不可用的UT西南校区的研究人员。为了适应活体动物、移植器官和组织切片的成像，我们要求将Zeiss LSM 710安装在AxioExaminer立式显微镜上，并配备两台Coherent Vision II Ti：Sapphire激光器。该系统被配置为允许同时进行双光子成像和双光子光活化、前向SHG的成像以及具有两个荧光通道的后向SHG的同时成像。我们需要一种GaAsP检测器，用于检测非常微弱的信号，例如在活体动物组织深处成像或在表达水平接近内源性水平的情况下成像荧光信号时出现的信号，例如在转基因动物中，其中转基因受内源性启动子控制。这项技术的加入将使与健康相关的研究项目成为可能，包括对大脑功能、免疫反应、伤口愈合、心脏病和早产的研究。

DESCRIPTION (provided by applicant): We are requesting an in vivo multiphoton microscope for the UT Southwestern Live Cell Imaging Facility (LCIF), a campus wide core facility that provides access to optical microscopy instrumentation for more than 150 researchers from 35 departments on the campus of UT Southwestern Medical Center in Dallas. This instrument will provide access to technology that currently is unavailable to researchers on the UT Southwestern campus. To accommodate imaging in live animals, explanted organs and tissue slices, we are requesting a Zeiss LSM 710 mounted on an AxioExaminer upright microscope with two Coherent Vision II Ti:Sapphire lasers. The system is configured to permit simultaneous two photon imaging and two photon photoactivation, imaging of forward directed SHG, and simultaneous imaging of backward directed SHG with two fluorescence channels. We are requesting a GaAsP detector for detecting very weak signals such as occur when imaging deep into tissue in live animals or imaging fluorescence signals where expression levels are near endogenous levels, such as in transgenic animals where the transgene is under the control of the endogenous promoter. The health-related research projects that will be enabled by the addition of this technology include studies of brain function, immune responses, wound healing, heart disease, and premature birth.