Flourescence-Activated Cell Sorter

描述(由申请人提供)：我们正在申请资金，将允许购买贝顿狄金森流入荧光激活的细胞分选机。该仪器将配置一个生物安全柜，允许对原代人类细胞群体以及已用各种病毒载体转导的原代细胞和细胞系进行分类和/或分析。该仪器还将配置为使用多达11种颜色和总共13个参数进行分类和/或执行多参数分析。本申请中描述的NIH资助的研究项目都需要使用具有这些功能的仪器。此外，该仪器将进一步支持我们扩大NIH资助的研究企业的努力。该仪器将允许坦普尔大学医学院基础科学系和临床系的研究人员开展至少28项NIH RO1拨款或计划项目中的工作。这款顶尖的多功能数字信号处理细胞分选仪将使对原代人类白细胞、神经前体细胞、内皮细胞、成骨细胞、树突状细胞、造血干细胞、各种实体肿瘤细胞群、白血病和许多转导细胞系进行流式细胞术成为可能。纳入的NIH资助的项目将使开展与提高我们对包括癌症、动脉粥样硬化在内的广泛疾病以及对包括艾滋病毒感染在内的一些传染病的抵抗力的广泛疾病的了解直接相关的研究成为可能。

DESCRIPTION (provided by applicant): We are requesting funds that will permit the purchase of a Becton Dickinson Influx Fluorescence Activated Cell Sorter. This instrument will be configured with a biological safety cabinet that will permit the sorting and/or analysis of primary human cell populations, as well as primary cells and cell lines, which have been transduced with various viral vectors. This instrument will also be configured to sort and/or perform multiparmetric analysis using up to 11 colors, and a total of 13 parameters. The NIH funded research projects described in this application all require access to an instrument that has these capabilities. In addition the instrument will further bolster our efforts to expand our NIH-funded research enterprise. This instrument will allow work to be carried out which is part of at least 28 NIH RO1 grants or Program Projects, by investigators in both basic science and clinical departments at Temple University School of Medicine. This top of the line versatile digital signal processing cell sorter will make it possible to carry out flow cytometry with primary human leukocytes, neuronal precursor cells, endothelial cells, osteoblasts, dendritic cells, hematopoietic stem cells, various solid tumor cell populations, leukemias, and a number of transduced cell lines. The NIH-funded projects that are included will make it possible to carry out research directly relevant to improving our understanding of a wide spectrum of diseases including cancer, atherosclerosis, and resistance to a number of infectious diseases, including infection with HIV.