BRONCHOALVEOLAR LAVAGE IN ASBESTOS EXPOSED INDIVIDUALS

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这项研究将确定是否在与石棉暴露一致的放射学异常的个体中，激活的肺泡巨噬细胞的数量增加，这些巨噬细胞释放的肽生长因子的数量增加，这些肽生长因子向间充质细胞发出增殖信号。 研究者将在mRNA水平测量巨噬细胞生长因子以评估基因表达，并测量巨噬细胞上清液以定量自发蛋白质释放。 该实验室用于以下方面：BAL分析、RNA分离、超离心、重组DNA技术、血液分离、ELISA和层流罩的使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study will determine if in individuals with radiographic abnormalties consistent with their asbestos exposure, there are increased numbers of activated alveolar macrophages which release increased quantities of peptide growth factors that signal mesenchymal cells to proliferate. The investigators will measure macrophage growth factors at the mRNA level to evaluate gene expression and macrophage supernatants to quantitate spontaneous protein release. The lab was used for the following: BAL analysis, RNA Isolation, ultracentrifugation, recombinant DNA techniques, blood separation, ELISA and use of laminar flow hoods.