Core B - Mutant Mouse

核心摘要 变异鼠核心(核心B)是所有三个项目都将广泛使用的基本核心。vt.给出 多种细胞类型上不同抑制受体之间的复杂相互作用，人们可以解剖的唯一方法 这些与体内疾病相关的关键生物学问题是通过复杂的小鼠模型来解决的。一个重要的 这个PPG应用程序的优势在于它将利用独特的免疫工具箱，特别是PD1/LAG3 小白鼠与特定血统的独特集合相结合，在大多数情况下是临时控制的， 表达Cre重组酶的小鼠。这些工具将给我们前所未有的控制，从而洞察 PD1/LAG3在多种细胞类型中的协同作用和功能。 目的1：提供所需的PD1/LAG3突变小鼠。该中心将由加州大学管理。 匹兹堡(匹兹堡)，大部分突变鼠的培育和采购外包给Taconic Under 小鼠无病原体屏障条件与他们的大多数商业育种实践相同。Taconic 将使用传统的递送机制每两周轮流将老鼠递送到所有研究地点， 从而避免了长时间的隔离。 目的2：产生新的突变小鼠组合。核心将产生新的突变小鼠 引入不同遗传因素的组合，如新的Cre菌株。 PPG互动：核心B将广泛支持项目1、项目2、项目3，并与核心互动 A便于获取育种、运输和基因分型数据。核心B还将执行额外的交叉 产生项目所需的任何菌株组合。核心B将与核心A交互，以提供轻松的 获得育种、运输和基因分型数据并进行持续监测。

CORE SUMMARY The Mutant Mouse Core (Core B) is an essential core that will be used extensively by all three projects. Given the complex interplay between different inhibitory receptors on multiple cell types, the only way one can dissect these critical disease-related biological questions in vivo is with sophisticated mouse models. A significant strength of this PPG application is the unique `immunological toolbox' that it will utilize, specifically PD1/LAG3 floxed mice combined with a unique collection of lineage-specific, and in most instances temporally controlled, Cre recombinase-expressing mice. These tools will give us unprecedented control and thus insight into PD1/LAG3 synergy and function in multiple cell types. AIM 1: To provide required PD1/LAG3 mutant mice. The Core will be administered from the University of Pittsburgh (Pitt), with the majority of the mutant mouse breeding and procurement outsourced to Taconic under Murine Pathogen Free barrier conditions identical to the majority of their commercial breeding practices. Taconic will deliver mice to all research sites on a rotating bi-weekly basis using their conventional delivery mechanism, thereby avoiding lengthy quarantine. AIM 2: To generate new mutant mouse combinations. The Core will generate new mutant mouse combinations that introduce different genetic elements such as new Cre stains. PPG Interactions: Core B will support Project 1, Project 2 and Project 3 extensively, and interact with Core A to provide easy access to breeding, shipping and genotyping data. Core B will also perform additional crosses to generate any strain combinations required by the projects. Core B will interact with Core A to provide easy access to breeding, shipping and genotyping data and ongoing monitoring.