Resource

摘要(资源) RATE资源和研究中心(RRRC)为 通过进口、储存和分发大量大鼠品系和胚胎干细胞的研究社区 台词。它确保有价值的模型被保存下来，并提供给感兴趣的调查人员，允许 研究人员满足NIH对资源共享的要求，减轻了个人调查人员的负担 动物分布，并确保模型保持严格的遗传质量控制和健康 监测标准，以提高实验的重复性。我们在下一个资助期的目标是 继续完善和扩大RRRC的功能和能力，为生物医学研究人员提供 通过大鼠模型、胚胎干细胞、相关试剂、方案和服务，将促进他们的 研究。我们将通过开发新技术和利用现有技术来提高质量来实现这一目标 资源的获取、超低温保存、储存、质量控制以及大鼠、精子、胚胎的分布 和胚胎干细胞。我们将继续评估和改进我们的服务提供和沟通 与生物医学研究团体合作。我们将扩展我们的服务，已经包括再衍生，殖民地 管理、广泛的遗传分析选项、使用CRISPR/Cas9技术的模型创建，以及 以收费服务为基础的微生物区系特征。通过这种方式，RRRC将增强其服务能力 为在生物医学研究中使用老鼠的研究人员提供亟需的“一站式”资源。

Abstract (Resource) The Rat Resource and Research Center (RRRC) provides a unique and essential repository service to the research community by importing, storing and distributing a large number of rat strains and embryonic stem cell lines. It assures that valuable models are preserved and made available to interested investigators, allows researchers to satisfy NIH requirements for resource sharing, relieves individual investigators from the burden of animal distribution and ensures that models are maintained with rigorous genetic quality control and health monitoring standards to enhance experimental reproducibility. Our objective during the next funding period is to continue to improve and expand the functions and capabilities of the RRRC to provide biomedical investigators with the rat models, embryonic stem cells, related reagents, protocols and services that will facilitate their research. We will accomplish this by developing new and utilizing existing technologies to improve the quality of the resource’s acquisition, cryopreservation, storage, quality control, and distribution of rats, sperm, embryos and embryonic stem cells. We will continue to evaluate and improve our service delivery and our communication with the biomedical research community. We will expand our services which already include re-derivation, colony management, a wide array of genetic analysis options, model creation using CRISPR/Cas9 technology, and microbiota characterization on a fee-for-service basis. In this way, the RRRC will strengthen its ability to serve as a much-needed “one stop” resource for investigators using rats in biomedical research.