Cell and Tissue Imaging

项目摘要：细胞和组织成像核心 先进的细胞显微镜是生物学研究的有力工具，在 糖尿病的发病机制和最终治疗的研究。成像技术已经发展到 在过去的十年中迅速提高了分辨率、灵敏度和速度，从而创造了新的 有机会在活细胞中实时研究许多数量级的过程 动物。然而，越来越复杂的设备的成本是巨大的，而专业知识 有效地使用、维护和开发这种设备在大多数实验室中并不常见。因此，有一种 这些强大工具的可用性与调查人员访问和使用这些工具的能力之间存在巨大差距 他们效率很高。响应DRC中心的反馈，建立了细胞和组织成像核心 成员利用在新成立的华盛顿大学中心的重大机构投资 为细胞成像(WUCCI)提供了一种研究结构和动态的集成方法 糖尿病相关细胞和组织的行为。核心的总体目标是提供访问 以及在使用先进细胞显微镜方面的技术支持，以加快步伐，扩大范围， 并提高华盛顿大学糖尿病研究的效率。核心服务满足独特的 众多研究人员对广泛的基础和翻译研究的要求，并吸引了新的 糖尿病研究的调查人员。重要的是，用户受益于深入的糖尿病和技术 核心董事和技术人员在咨询实验设计和设计时所用的专业知识 数据的解释。在上一个供资周期中，该中心为67名刚果民主共和国调查员研究提供了服务 实验室(包括14名P&F接受者)，他们在核心充值方面花费了788,342美元，代表 约占所有WUCCI活动的18%。18个刚果民主共和国成员实验室获得小额赠款资助 通过DRC成像核心奖学金计划。这些实验室中有10个是由初级教师指导的 助理教授职级的成员。使用和引用核心服务的出版物有35篇。给定 在过去的供资周期中广泛使用核心，并建立新的成像技术(例如 冷冻-EM和晶格光刻)、样品制备服务(例如组织清理)和分析方法 (例如，基于人工智能的免疫组织化学和免疫荧光图像分析)，预计请求 细胞和组织成像核心服务将继续增长。刚果民主共和国成员对以下方面的大量需求 先进的显微成像和图像分析是细胞和组织成像核心的有力证据 提供利用慷慨的机构支持和独特的专业知识的服务，以促进重要和 创新的糖尿病研究。

PROJECT SUMMARY: Cell and Tissue Imaging Core Advanced cellular microscopy is a powerful tool for biological research and plays an important role in the study of the pathogenesis and eventual treatment of diabetes mellitus. Imaging technology has evolved rapidly over the last decade leading to improvements in resolution, sensitivity and speed that have created new opportunities for studying processes across many orders of magnitude and in real-time in living cells and animals. However, the costs of increasingly sophisticated equipment are substantial and the expertise to efficiently use, maintain, and develop this equipment is not common in most labs. There is therefore a significant gap between the availability of these powerful tools and the ability of investigators to access and use them efficiently. The Cell and Tissue Imaging Core was established in response to feedback from DRC center members to leverage the significant institutional investment in the newly created Washington University Center for Cellular Imaging (WUCCI) by providing an integrated approach to investigate the structure and dynamic behavior of diabetes-related cells and tissues. The overall objective of the Core is to provide access to and technical support in using advanced cellular microscopy to accelerate the pace, expand the scope, and improve the efficiency of diabetes research at Washington University. Core services meet the unique requirements of numerous investigators over a wide range of basic and translational research and attract new investigators into diabetes research. Importantly, users benefit from the in-depth diabetes and technical expertise of the Core directors and technical staff in time spent in consultation for experimental design and interpretation of data. During the past funding cycle, the Core served 67 DRC investigator research laboratories (including 14 P&F recipients), who expensed $788,342 in core recharges, representing approximately 18% of all WUCCI activity. Eighteen DRC member laboratories received micro-grant funding through the DRC Imaging Core Scholarship program. Ten of these labs were directed by junior faculty members at the rank of Assistant Professor. Thirty-five publications used and cited core services. Given the broad use of the Core over the past funding cycle, and the establishment of new imaging technologies (e.g. cryo-EM and lattice lightsheet), sample preparation services (e.g. tissue clearing), and analysis approaches (e.g. AI-based immunohistochemistry and immunofluorescent image analysis), it is anticipated that requests for Cell and Tissue Imaging Core services will continue to grow. The substantial demand by DRC members for advanced microscopic imaging and image analysis is strong evidence that the Cell and Tissue Imaging Core provides services that leverage generous institutional support and unique expertise to facilitate important and innovative diabetes research.