Translational Biomarker Analytical Core

转化型生物标志物分析核心(TBAC)实验室向DRC提供 研究人员使用分析技术支持糖尿病、胰岛素抵抗、 糖尿病在人类受试者和动物中的中间代谢和医学后果 模特们。所提供的服务最好设在核心实验室，因为它们需要调查人员 难以或不切实际的咨询、特殊手段和方法 单独的实验室。此外，这种安排允许协调注意可靠性和 所用分析方法的重现性。在研究葡萄糖动态平衡，胰岛素分泌， 胰岛素抵抗，血脂异常，激素作用，分子遗传学，或药物效应，它是必不可少的 能够监测胰岛素、C-肽、胰高血糖素、GLP-1、GIP、PYY、皮质醇和 儿茶酚胺以及葡萄糖、游离脂肪酸、甘油、酮、氨基酸、脂类等代谢物 作为更详细的代谢组学指纹评估。随着越来越多的技术人员 开发和验证新的疾病生物标记物的能力，核心的关键功能之一是 尽快开发和创新这些新方法，使研究基地能够发挥作用 在这些化验中。TBAC的具体目标是：1)满足对动态的高需求 研究基地的生物检测范围，提高效率、质量和重复性，同时 最大限度地降低成本。2)支持发展新的化验方法，以回应会员的需要 以及利用核心员工的专业知识的方法。3)促进访问有针对性和 哥伦比亚欧文生物标记实验室的非靶向代谢组学/脂质组学测定和 新近扩展的代谢组学核心。4)为了方便接触生物信息学专家，动力学 正确解释代谢组学/脂质组学所需的研究和途径分析 测定和稳定同位素动力学研究。5)向调查人员提供专家意见和 他们在分析方法、实验设计和最先进的数据解释方面的同事 糖尿病的表型。6)对初级研究员和博士后研究员进行以下方面的实用技能培训 核心方法，并在适当时将分析方法转移到自己的实验室。7)以促进 研究基地之间的合作，宣传可用的资源，开发和/或促进 研究人员获得新技术，如基于MS的分析和量化方法 代谢物。TBAC的分析和咨询能力与其他刚果民主共和国密切相关 为定量代谢分析提供综合方法的核心。

The Translational Biomarker Analytical Core (TBAC) Laboratory makes available to DRC investigators analytical techniques to support studies of diabetes mellitus, insulin resistance, intermediary metabolism, and medical consequences of diabetes in human subjects and animal models. The services provided are best based in a Core Laboratory because they require investigator consultation, special instrumentation and methods that are difficult or impracticable to establish in individual laboratories. In addition, this arrangement allows for coordinated attention to reliability and reproducibility of the assays employed. In investigations of glucose homeostasis, insulin secretion, insulin resistance, dyslipidemia, hormone action, molecular genetics, or drug effects, it is essential to be able to monitor hormones such as insulin, C-peptide, glucagon, GLP-1, GIP, PYY, cortisol and catecholamines and such metabolites as glucose, FFA, glycerol, ketones, amino acids, lipids, as well as more detailed assessment of the metabolomics fingerprint. With the increasing technical capabilities to develop and validate novel disease biomarkers, one of the key functions of the Core is to expeditiously develop and inaugurate these new methods so that the research base can avail itself of these assays. The SPECIFIC AIMS of the TBAC are: 1) To meet the high demand for a dynamic range of bioassays by the research base, enhancing efficiency, quality, and reproducibility, while minimizing costs. 2) To respond to members’ needs by supporting the development of new assays and methods that leverage the expertise of the Core staff. 3) To facilitate access to targeted and untargeted metabolomics/lipidomics determinations by the Columbia Irving Biomarker Laboratory and the newly expanded Metabolomics core. 4) To facilitate access to experts in bioinformatics, kinetic studies and pathway analyses required to appropriately interpret metabolomics/lipidomics determinations and stable isotopes kinetic studies. 5) To provide expert advice to investigators and their associates on analytical methods, experimental design, and data interpretation for state-of-the art phenotyping of diabetes. 6) To train junior investigators and post-doctoral fellows in practical skills in the Core methods and, if appropriate, transfer analytical methods to their own labs.7) To facilitate collaborations among the research base, advertise available resources, and develop and/or facilitate investigator access to new technologies, such as MS-based methods to analyze and quantify metabolites. TBAC analytic and consultative capabilities are closely articulated with the other DRC Cores to provide an integrated approach to quantitative metabolic analyses.