Nutrition and Physical Activity Assessment Study - Assessment of Dietary Supplements

摘要 我们正在回应PA-20-227“膳食补充剂研究的行政补充”， 支持对先前收集关于我们的母基金R 01 CA 119171的数据进行饮食依从性相关分析， 营养和体力活动评估研究（NPAAS）。NPAAS父母补助金的目标是 发现和利用新的、可靠的营养物质、食物和饮食模式的生物标志物，以加强 营养流行病学和理解饮食相关的慢性疾病风险。在以往的融资周期中， 实施饮食评估、双标记水（DLW）和生物标本采集协议（2006- 2010年）在妇女健康倡议（WHI）观察性研究（NPAAS-OS）的450名参与者中。我们接下来 在153名WHI参与者中进行了一项新的控制喂养研究（2011-2014），其中每位参与者 为她提供了一种接近于平时饮食的饮食，这样血液和尿液的测量值就能迅速稳定下来， 研究人群的摄入量变化将被保留，我们实施了DLW和生物标本 在这两项研究中，空腹血和尿（24小时和24小时） 斑点）分析营养素（B-12、叶酸、α-和β-胡萝卜素、叶黄素+玉米黄质、番茄红素、α- 和γ-生育酚）和代谢组学的3个平台：GC-MS，LC-MS和NMR。膳食补充剂 还收集了摄入量数据; NPAAS-OS中68%的妇女和NPAAS-FS中88%的妇女报告使用 补充剂.本行政补编拟议的工作将利用这些先前的 收集的数据。补编的具体目标是：）从开放式文本中创建构造变量 NPAAS-FS多日食品记录中的实地膳食补充剂记录; 2）描述常见的 NPAAS参与者的膳食补充剂暴露类别，使用新构建的变量， NPAAS-FS和NPAAS-OS; 3）描述特定类别膳食补充剂的贡献 使用NPAAS-FS中消耗的营养素和膳食生物标志物之间的整体关联，并应用 NPAAS-OS中的模型;以及4）评估已建立的营养物生物标志物和 血清和尿液代谢组学特征，以表征从食物和膳食补充剂中摄入的营养素， 到目前为止，NPAAS-FS和NPAAS-OS的膳食补充剂数据已经 没有完全分类。将补充数据进一步分类为可用类别（单个 补充剂，多种成分，其他组合）可能很重要，因为我们在NPAAS的初步工作- FS表明，二元（是/否）膳食补充剂使用变量是分析的重要预测因素， 浓度生物标志物和营养摄入之间的关联。只使用一个二进制变量， 目的非常有限。作为回应，本行政补充文件将导致对总养分的估计， 暴露于食物+补充剂，从而增强营养生物标志物的发现、验证和 应用程序.这些目标与父母补助金的目标完全一致。

ABSTRACT We are responding to PA-20-227 “Administrative Supplement for Research on Dietary Supplements” to support dietary supplement-related analysis of previously collected data on our parent grant R01 CA119171, “Nutrition and Physical Activity Assessment Study,” (NPAAS). The goal of the NPAAS parent grant is to discover and utilize novel, reliable biomarkers of nutrients, foods and dietary patterns to enhance the field of nutritional epidemiology and the understanding diet-related chronic disease risk. In previous funding cycles we implemented dietary assessment, doubly labeled water (DLW) and biospecimen collection protocols (2006- 2010) in 450 participants in the Women’s Health Initiative (WHI) Observational Study (NPAAS-OS). We next conducted a novel controlled feeding study in 153 WHI participants (2011-2014), in which each participant was provided a diet approximating her usual diet so that blood and urine measures would stabilize quickly and intake variations in the study population would be preserved, and we implemented the DLW and biospecimen collection protocols in NPAAS-FS to match NPAAS-OS. In both studies, fasting blood and urine (24-h and spot) were analyzed for nutrients (B-12, folate, alpha- & beta-carotene, lutein + zeaxanthin, lycopene, alpha- and gamma tocopherol) and metabolomics by 3 platforms: GC-MS, LC-MS and NMR. Dietary supplement intake data were also collected; 68% of women in the NPAAS-OS and 88% in the NPAAS-FS reported using supplements. The work proposed for this Administrative Supplement will make use of these previously collected data. The Supplement specific aims are: ) To create constructed variables from the open-ended text field dietary supplement records in the NPAAS-FS multiple day food records; 2) To describe common categories of dietary supplement exposure in NPAAS participants using the new constructed variables from NPAAS-FS and NPAAS-OS; 3) To characterize the contribution of specific categories of dietary supplement use to the overall associations between consumed nutrients and dietary biomarkers in NPAAS-FS and to apply the models in NPAAS-OS; and 4) To evaluate the utility of combinations of established nutrient biomarkers and serum and urine metabolomics profiles to characterize nutrient intakes from food and dietary supplements in the NPAAS-FS and NPAAS-OS. To date, the dietary supplement data for NPAAS-FS and NPAAS-OS have not been fully categorized. Further classification of the supplement data into useable categories (single supplements, multi-ingredients, other combinations) may be important because our initial work in the NPAAS- FS showed that a binary (yes/no) dietary supplement use variable was an important predictor in analyses of the association between concentration biomarkers and nutrient intake. Use of only a binary variable for such a purpose is very limiting. In response, this Administrative Supplement will lead to estimates of total nutrient exposure from food + supplements thereby enhancing nutritional biomarker discovery, validation and application. These goals align well with the parent grant’s aims.