P30 Center Core Grant for Vision Research

摘要 细胞成像核心 俄克拉荷马州大学健康科学中心的视觉研究实验室的一个关键重点是 在细胞水平上理解眼组织生理学和病理生理学。每一个视觉研究 我们校园的实验室需要必要的基础设施和培训，以标记，成像和定量 在细胞和亚细胞水平上分析组织。此外，我们研究人员的竞争力 要求他们将新的成像模式、分析方法和硬件纳入研究 程序.培训和获得最先进的细胞成像模式是昂贵的， 共享的核心设施，以促进创新的新研究方法和合作，以维持高 生产力，并保持在该领域的最前沿。细胞成像和形态分析核心 (CIC)目前支持16个NEI资助的R01赠款的视觉研究，提供知识渊博的专家 支持沿着的明场、落射荧光、体视显微镜和激光扫描共焦 显微镜用于在宏观和微观水平上进行图像捕获。高度复杂的软件包 CIC提供的Metamorph和Imaris使视觉研究人员能够渲染和分析图像。的 核心为学生，博士后研究员，工作人员和校长提供高质量的培训 调查人员使用建造及工业督察的设备和资源，以大大扩展其调查能力， 发展创意，促进合作互动。

ABSTRACT Cellular Imaging Core A crucial focus of vision research laboratories at the University of Oklahoma Health Sciences Center is understanding ocular tissue physiology and pathophysiology at the cellular level. Every vision research laboratory on our campus requires the infrastructure and training necessary to label, image, and quantitatively analyze tissues at the cellular and subcellular levels. Furthermore, the competitiveness of our researchers requires that they incorporate new imaging modalities, analytical approaches, and hardware into their research programs. Training on and access to state-of-the-art cellular imaging modalities is expensive and necessitates shared core facilities to foster innovative new research approaches and collaborations, to sustain high productivity, and remain at the forefront of the field. The Cellular Imaging and Morphometric Analysis Core (CIC) currently supports vision research on 16 NEI-funded R01 grants, providing highly knowledgeable expert support along with the bright field, epifluorescence, stereomicroscopes and laser scanning confocal microscopes for image capture at the macro and micro levels. The highly sophisticated software packages provided by the CIC (Metamorph and Imaris) enable vision researchers to render and analyze images. The core provides access and high quality training for students, postdoctoral fellows, staff, and principal investigators on the use of CIC equipment and resources to greatly expand their investigative capabilities, develop creative new ideas, and promote collaborative interactions.