Geroscience Pathology and Cellular Histology

核心D：总结Ikeno 为了确定实验性干预对衰老的影响，研究人员必须 了解干预如何改变随年龄增长而发生的病理损害。病理增加 随着年龄的增长呈指数增长，并在很大程度上导致与年龄有关的发病率和死亡率。病理性的 信息也为研究人员提供了对可能的生物/分子机制的洞察(S)。 干预。此外，对老动物的病理评估是必要的，以帮助调查人员确定 所测量的生理和生化参数的变化是否与 与任何潜在的病理情况无关。此外，幼年动物的病理分析 为调查人员提供有关基因和药物干预影响 发展。因此，获得准确和彻底的老年人衰老的病理评估是至关重要的。 和幼小的动物。老年科学病理学和细胞组织学核心(GPCHC)将提供项目 研究人员对动物随着年龄增长而发生的损伤进行了详细的病理分析。GPCHC将 还为研究人员提供细胞衰老特征、组织病理学、形态计量学和 特定病变和组织(例如，肿瘤性病变、肾小球肾炎、 脑内胶质细胞增生症、胰岛数目和大小、巨噬细胞浸润和脂肪细胞形态 脂肪组织)从被研究的小鼠身上获得。此外，GPCHC将开发一个全面的数据库 组织病理学数据和图像作为组织病理学幻灯片的来源：1)由 调查人员；和2)包含石蜡和冰冻块的组织档案，用于应要求分析样本 并进行新的形态研究。此外，GPCHC将为研究人员提供组织学方面的 支持。我们将准备石蜡包埋和冰冻切片，制作未染色的石蜡或冰冻切片，以及 进行各种组织学染色技术，包括特殊染色。 这些数据将使研究人员能够评估遗传和药物操纵对 潜在致命损害和年龄敏感特征的发展，并提供信息是否 在这些项目中观察到的生化/分子变化与组织病理学变化有关 特定的组织。

CORE D: SUMMARY Ikeno In order to determine the impact of an experimental intervention on aging, it is essential that investigators understand how an intervention alters pathological lesions that occur with age. Pathology increases exponentially with advancing age and is largely responsible for age-related morbidity and mortality. Pathological information also provides investigators with insight into the potential biological/molecular mechanism(s) of the intervention. Furthermore, pathological assessment of old animals is necessary to help investigators determine whether changes in the physiological and biochemical parameters measured are associated with or are independent of any underlying pathological conditions. In addition, pathological analysis of young animals provides investigators with information about genetic and pharmacological interventions that affect development. Thus, it is essential to obtain accurate and thorough pathological assessments of aging in both old and young animals. The Geroscience Pathology and Cellular Histology Core (GPCHC) will provide Project investigators with detailed pathological analyses of lesions that occur with age in the animals. The GPCHC will also provide investigators with cellular senescence characterization, histopathological, morphometric, and immunohistochemical analyses of specific lesions and tissues (e.g., neoplastic lesions, glomerulonephritis, gliosis in the brain, number and size of pancreatic islets, macrophage infiltration, and fat cell morphology in adipose tissue) obtained from the mice studied. In addition, the GPCHC will develop a comprehensive database of histopathological data and images of histopathology slides as a resource for: 1) trend analyses by the investigators; and 2) a tissue archive containing paraffin and frozen blocks for analysis of samples by request and for new morphological research. Furthermore, the GPCHC will provide investigators with histological support. We will prepare paraffin-embedded and frozen blocks, make unstained paraffin or frozen sections, and perform various histological staining techniques, including special staining. These data will allow investigators to evaluate the effects of genetic and pharmacological manipulations on the development of potentially fatal lesions and age-sensitive traits, and also provide information on whether the biochemical/molecular changes observed in the projects are correlated to the histopathological changes in specific tissues.