Spatial in situ mapping of RNA-chromatin interactions at transcriptome-and-genome scale in human tissues

人类血管中RNA-染色质相互作用的空间原位定位 摘要 该项目的一个突破口是同时提高了最先进的空间分辨率、动态范围 RNA的量化，以及人体组织的适用尺寸增加了一到几个数量级。这 突破将为用户提供极高分辨率、高密度、高保真和可重现的空间 测绘幻灯片(高保真幻灯片)，用于空间转录组分析或修饰或相互作用的空间测绘 核酸。我们将利用高保真幻灯片来提供空间转录组和空间RNA染色质 以前所未有的分辨率提供互动组地图工具。该项目将产生高保真空间转录组， HIFI空间RNA-crhomatin相互作用组和96个蛋白质的高分辨蛋白质空间图谱 静脉的肠系膜动脉，胰腺、肝脏和脑的微血管。这些数据集将形成 最初的人类EC生物分子图谱(HuEC-MAP)。这些数据将提供前所未有的洞察力 血管内皮细胞特定的组织形态和功能的分子性质，它们是 对相关组织的发育和功能至关重要。

Spatial in situ mapping of RNA-chromatin interactions in human vasculature Abstract A breakthrough of this project is to simultaneously improve the state-of-art spatial resolution, dynamic range of RNA quantification, and applicable dimensions of human tissue by one to several orders of magnitude. This breakthrough will deliver to users extremely high-resolution, high-density, high-fidelity and reproducible spatial mapping slides (HiFi slides) for spatial transcriptome analysis or spatial mapping of modified- or interacting- nucleic acids. We will leverage HiFi slides to provide spatial transcriptome and spatial RNA-chromatin interactome mapping tools in unprecedented resolution. This project will generate HiFi spatial transcriptome, HiFi spatial RNA-crhomatin interactome, and high-resolution protein spatial profiles of 96 proteins from mesenteric arteries of veins, and the microvasculature from pancreas, liver and brain. These datasets will form the initial human EC biomolecular map (HuEC-MAP). These data will offer unprecedented insights to the molecular nature of the tissue-specific morphologies and functions of the blood vessel endothelium, which are critical to the development and functions of the associated tissues.