Organoid and Minibioreactor Array Cultivation Core

项目摘要 U19传染病基因组中心（GCID）应用程序的目标之一是使用最先进的 艺术平台，以解决围绕多种NIAID A-C类细菌，病毒，真菌， 以及引起胃肠道、肠外和呼吸道疾病的寄生粘膜病原体。到 为了实现这一目标，建立类器官和微型生物反应器阵列培养（OMAC）核心将允许 提供关键的生物学相关的人类器官型和细菌培养物， 项目的成功。核心将围绕三个目标进行组织：1）生成，维护和提供 实验准备肠道和气道类器官培养物和专用生长试剂，（2） 培养来自肠和肺的各种类型的微生物群落，以及（3）建立和监督 利用厌氧平台进行实验，以促进微生物和类器官的共培养。这些目标将 通过体外验证获得的关键结果，提供翻译基因组分析的能力， 测序研究。核心将减少劳动力，供应和设备成本，以及完成所需的时间 通过集中分离，生长和维持类器官系和复杂的微生物， 社区.培养类器官和细菌所需材料的批量获取， 含有生长因子的条件培养基和生物反应器培养基，以及 生产线和生物反应器为各个项目研究人员节省了大量时间和成本， 确保标准化和再现性。此外，OMAC核心将增强通信， 项目之间取得的成果的可比性。OMAC核心将是 成功的每一个项目在这个应用程序以及一个统一的元素，在连接它们在一起。

PROJECT SUMMARY One of the goals of this U19 Genomic Center for Infectious Disease (GCID) application is to use state-of-the- art platforms to address gaps in knowledge surrounding multiple NIAID Category A-C bacterial, viral, fungal, and parasitic mucosal pathogens that cause gastrointestinal, extraintestinal, and respiratory tract diseases. To meet this goal, the establishment of the Organoid and Minibioreactor Array Cultivation (OMAC) core will allow the provision of critical biologically relevant human organotypic and bacterial cultures that are essential for the success of the project. The core will be organized around three goals: 1) generate, maintain, and provide experimentally ready intestinal and airway organoid cultures and specialized growth reagents for use, (2) cultivate various types of microbial communities from the intestine and lung, and (3) establish and oversee experiments with an anaerobic platform to facilitate co-culture of microbes and organoids. These goals will offer the ability to translate genomic analysis through in vitro validation of key outcomes obtained from sequencing studies. The core will reduce labor, supply and equipment costs, and time required to complete projects by centralizing the isolation, growth, and maintenance of the organoid lines and complex microbial communities. Bulk acquisition of materials required to culture organoids and bacteria, standardization of conditioned media containing growth factors and bioreactor media, and centralization of the maintenance of the lines and bioreactors results in significant time and cost savings to the individual Project investigators and ensures standardization and reproducibility. Additionally, the OMAC core will enhance communication and comparability of results obtained between projects. The OMAC core will be an essential component of the success of each of the projects in this application as well as a unifying element in connecting them together.